| Literature DB >> 31504824 |
Shiqi Mao1, Yachen Ying1, Xiaotian Wu1,2, Christopher J Krueger1,3, Antony K Chen1.
Abstract
Clustered regularly interspaced short palindromic repeats (CRISPR)-based genomic imaging systems predominantly rely on fluorescent protein reporters, which lack the optical properties essential for sensitive dynamic imaging. Here, we modified the CRISPR single-guide RNA (sgRNA) to carry two distinct molecular beacons (MBs) that can undergo fluorescence resonance energy transfer (FRET) and demonstrated that the resulting system, CRISPR/dual-FRET MB, enables dynamic imaging of non-repetitive genomic loci with only three unique sgRNAs.Entities:
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Year: 2019 PMID： 31504824 PMCID： PMC6847002 DOI： 10.1093/nar/gkz752
Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971