Literature DB >> 28994406

Crystallization via tubing microfluidics permits both in situ and ex situ X-ray diffraction.

Charline J J Gerard1, Gilles Ferry2, Laurent M Vuillard2, Jean A Boutin2, Leonard M G Chavas3, Tiphaine Huet3, Nathalie Ferte1, Romain Grossier1, Nadine Candoni1, Stéphane Veesler1.   

Abstract

A microfluidic platform was used to address the problems of obtaining diffraction-quality crystals and crystal handling during transfer to the X-ray diffractometer. Crystallization conditions of a protein of pharmaceutical interest were optimized and X-ray data were collected both in situ and ex situ.

Keywords:  crystallization; ex situ X-ray diffraction; in situ X-ray diffraction; microfluidics; optimization

Mesh：

Year:  2017        PMID： 28994406      PMCID： PMC5633925          DOI： 10.1107/S2053230X17013826

Source DB:  PubMed          Journal:  Acta Crystallogr F Struct Biol Commun        ISSN： 2053-230X            Impact factor:   1.056


  20 in total

Review 1.  The promise of macromolecular crystallization in microfluidic chips.

Authors:  Mark van der Woerd; Darren Ferree; Marc Pusey
Journal:  J Struct Biol       Date:  2003-04       Impact factor: 2.867

2.  ExPASy: The proteomics server for in-depth protein knowledge and analysis.

Authors:  Elisabeth Gasteiger; Alexandre Gattiker; Christine Hoogland; Ivan Ivanyi; Ron D Appel; Amos Bairoch
Journal:  Nucleic Acids Res       Date:  2003-07-01       Impact factor: 16.971

3.  Nanoliter microfluidic hybrid method for simultaneous screening and optimization validated with crystallization of membrane proteins.

Authors:  Liang Li; Debarshi Mustafi; Qiang Fu; Valentina Tereshko; Delai L Chen; Joshua D Tice; Rustem F Ismagilov
Journal:  Proc Natl Acad Sci U S A       Date:  2006-12-11       Impact factor: 11.205

4.  A microfluidic device for kinetic optimization of protein crystallization and in situ structure determination.

Authors:  Carl L Hansen; Scott Classen; James M Berger; Stephen R Quake
Journal:  J Am Chem Soc       Date:  2006-03-15       Impact factor: 15.419

5.  In situ data collection and structure refinement from microcapillary protein crystallization.

Authors:  Maneesh K Yadav; Cory J Gerdts; Ruslan Sanishvili; Ward W Smith; L Spencer Roach; Rustem F Ismagilov; Peter Kuhn; Raymond C Stevens
Journal:  J Appl Crystallogr       Date:  2005-12       Impact factor: 3.304

6.  Microfluidic crystallization.

Authors:  Jacques Leng; Jean-Baptiste Salmon
Journal:  Lab Chip       Date:  2008-10-24       Impact factor: 6.799

7.  Crystal structure of human quinone reductase type 2, a metalloflavoprotein.

Authors:  C E Foster; M A Bianchet; P Talalay; Q Zhao; L M Amzel
Journal:  Biochemistry       Date:  1999-08-03       Impact factor: 3.162

8.  Identification of the melatonin-binding site MT3 as the quinone reductase 2.

Authors:  O Nosjean; M Ferro; F Coge; P Beauverger; J M Henlin; F Lefoulon; J L Fauchere; P Delagrange; E Canet; J A Boutin
Journal:  J Biol Chem       Date:  2000-10-06       Impact factor: 5.157

9.  Quinone reductase 2 is a catechol quinone reductase.

Authors:  Yue Fu; Leonid Buryanovskyy; Zhongtao Zhang
Journal:  J Biol Chem       Date:  2008-06-24       Impact factor: 5.157

10.  Microfluidic chips for the crystallization of biomacromolecules by counter-diffusion and on-chip crystal X-ray analysis.

Authors:  Kaouthar Dhouib; Chantal Khan Malek; Wilhelm Pfleging; Bernard Gauthier-Manuel; Roland Duffait; Gaël Thuillier; Rosaria Ferrigno; Lilian Jacquamet; Jeremy Ohana; Jean-Luc Ferrer; Anne Théobald-Dietrich; Richard Giegé; Bernard Lorber; Claude Sauter
Journal:  Lab Chip       Date:  2009-03-02       Impact factor: 6.799
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