Rossella Mazzilli1, Danilo Cimadomo2, Alberto Vaiarelli3, Antonio Capalbo4, Lisa Dovere5, Erminia Alviggi6, Ludovica Dusi7, Carlo Foresta8, Francesco Lombardo9, Andrea Lenzi9, Herman Tournaye10, Carlo Alviggi11, Laura Rienzi12, Filippo Maria Ubaldi12. 1. Andrology Unit, Department of Clinical and Molecular Medicine, Sant'Andrea Hospital, University of Rome "Sapienza," Rome, Italy; Clinica Valle Giulia, Genera Centers for Reproductive Medicine, Rome, Italy. Electronic address: rossella.mazzilli@uniroma1.it. 2. Clinica Valle Giulia, Genera Centers for Reproductive Medicine, Rome, Italy; Dipartimento di Scienze Anatomiche, Istologiche, Medico-Legali e dell'Apparato Locomotore, Sezione Istologia ed Embriologia Medica, "Sapienza," University of Rome, Rome, Italy. 3. Clinica Valle Giulia, Genera Centers for Reproductive Medicine, Rome, Italy; Department of Human Pathology in Adulthood and Childhood "G. Barresi," University of Messina, Messina, Italy. 4. Clinica Valle Giulia, Genera Centers for Reproductive Medicine, Rome, Italy; Genetyx, Molecular Biology Laboratories, Marostica (VI), Italy. 5. Clinica Valle Giulia, Genera Centers for Reproductive Medicine, Rome, Italy. 6. Ruesch Clinic, Genera Centers for Reproductive Medicine, Naples, Italy. 7. Poliambulatorio Salus, Genera Centers for Reproductive Medicine, Marostica (VI), Italy. 8. Department of Medicine, Unit of Andrology and Reproductive Medicine, University of Padova, Padova, Italy. 9. Department of Experimental Medicine, "Sapienza" University of Rome, Rome, Italy. 10. Center for Reproductive Medicine, Universitair Ziekenhuis Brussel, Brussels, Belgium. 11. Department of Neuroscience, Reproductive Science and Odontostomatology, University of Naples Federico II, Naples, Italy. 12. Clinica Valle Giulia, Genera Centers for Reproductive Medicine, Rome, Italy; Ruesch Clinic, Genera Centers for Reproductive Medicine, Naples, Italy; Poliambulatorio Salus, Genera Centers for Reproductive Medicine, Marostica (VI), Italy.
Abstract
OBJECTIVE: To evaluate the impact of the male factor on the outcomes of intracytoplasmic sperm injection (ICSI) cycles combined with preimplantation genetic testing for aneuploidies (PGT-A). DESIGN: Observational longitudinal cohort study. SETTING: Private in vitro fertilization (IVF) center. PATIENT(S): A total of 1,219 oocyte retrievals divided into five study groups according to sperm parameters: normozoospermia (N), moderate male factor (MMF), severe oligoasthenoteratozoospermia (OAT-S), obstructive azoospermia (OA), and nonobstructive azoospermia (NOA). INTERVENTION(S): ICSI with ejaculated/surgically retrieved sperm, blastocyst culture, trophectoderm-based quantitative polymerase chain reaction PGT-A, and frozen-warmed euploid embryo transfer (ET). MAIN OUTCOMES MEASURE(S): The primary outcome measures were fertilization, blastocyst development, and euploidy rates; the secondary outcome measures were live birth and miscarriage rates. Perinatal and obstetrical outcomes were monitored as well. RESULT(S): A total of 9,042 metaphase II oocytes were inseminated. The fertilization rate was significantly reduced in MMF, OAT-S, OA, and NOA compared with N (74.8%, 68.7%, 67.3%, and 53.1% vs. 77.2%). The blastocyst rate per fertilized oocyte was significantly reduced in MMF and NOA compared with N (48.6% and 40.6% vs. 49.3%). The timing of blastocyst development also was affected in OA and NOA. Logistic regression analysis adjusted for confounders highlighted NOA as a negative predictor of obtaining an euploid blastocyst per OPU (odds ratio 0.5). When the analysis was performed per obtained blastocyst, however, no correlation between male factor and euploidy rate was observed. Embryo transfers also resulted in similar live birth and miscarriage rates. No impact of sperm factor on obstetrical/perinatal outcomes was observed. CONCLUSION(S): Severe male factor impairs early embryonic competence in terms of fertilization rate and developmental potential. However, the euploidy rate and implantation potential of the obtained blastocysts are independent from sperm quality.
OBJECTIVE: To evaluate the impact of the male factor on the outcomes of intracytoplasmic sperm injection (ICSI) cycles combined with preimplantation genetic testing for aneuploidies (PGT-A). DESIGN: Observational longitudinal cohort study. SETTING: Private in vitro fertilization (IVF) center. PATIENT(S): A total of 1,219 oocyte retrievals divided into five study groups according to sperm parameters: normozoospermia (N), moderate male factor (MMF), severe oligoasthenoteratozoospermia (OAT-S), obstructive azoospermia (OA), and nonobstructive azoospermia (NOA). INTERVENTION(S): ICSI with ejaculated/surgically retrieved sperm, blastocyst culture, trophectoderm-based quantitative polymerase chain reaction PGT-A, and frozen-warmed euploid embryo transfer (ET). MAIN OUTCOMES MEASURE(S): The primary outcome measures were fertilization, blastocyst development, and euploidy rates; the secondary outcome measures were live birth and miscarriage rates. Perinatal and obstetrical outcomes were monitored as well. RESULT(S): A total of 9,042 metaphase II oocytes were inseminated. The fertilization rate was significantly reduced in MMF, OAT-S, OA, and NOA compared with N (74.8%, 68.7%, 67.3%, and 53.1% vs. 77.2%). The blastocyst rate per fertilized oocyte was significantly reduced in MMF and NOA compared with N (48.6% and 40.6% vs. 49.3%). The timing of blastocyst development also was affected in OA and NOA. Logistic regression analysis adjusted for confounders highlighted NOA as a negative predictor of obtaining an euploid blastocyst per OPU (odds ratio 0.5). When the analysis was performed per obtained blastocyst, however, no correlation between male factor and euploidy rate was observed. Embryo transfers also resulted in similar live birth and miscarriage rates. No impact of sperm factor on obstetrical/perinatal outcomes was observed. CONCLUSION(S): Severe male factor impairs early embryonic competence in terms of fertilization rate and developmental potential. However, the euploidy rate and implantation potential of the obtained blastocysts are independent from sperm quality.
Authors: A M Mahesan; S Sadek; V Moussavi; T Vazifedan; A Majeed; T Cunningham; S Oehninger; S Bocca Journal: J Assist Reprod Genet Date: 2018-06-20 Impact factor: 3.412
Authors: Danilo Cimadomo; C Scarica; R Maggiulli; G Orlando; D Soscia; L Albricci; S Romano; F Sanges; F M Ubaldi; L Rienzi Journal: J Assist Reprod Genet Date: 2018-05-03 Impact factor: 3.412
Authors: Luis R Hoyos; Connie Y Cheng; Kathleen Brennan; Gary Hubert; Brandon Wang; Richard P Buyalos; Molly Quinn; Mousa Shamonki Journal: J Assist Reprod Genet Date: 2020-01-18 Impact factor: 3.412