Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 CRISPR/Cas9-Based Engineering of the Epigenome.

Literature DB >> 28985525

CRISPR/Cas9-Based Engineering of the Epigenome.

Julian Pulecio¹, Nipun Verma², Eva Mejía-Ramírez³, Danwei Huangfu⁴, Angel Raya⁵.

Abstract

Determining causal relationships between distinct chromatin features and gene expression, and ultimately cell behavior, remains a major challenge. Recent developments in targetable epigenome-editing tools enable us to assign direct transcriptional and functional consequences to locus-specific chromatin modifications. This Protocol Review discusses the unprecedented opportunity that CRISPR/Cas9 technology offers for investigating and manipulating the epigenome to facilitate further understanding of stem cell biology and engineering of stem cells for therapeutic applications. We also provide technical considerations for standardization and further improvement of the CRISPR/Cas9-based tools to engineer the epigenome.

Entities: CellLine Chemical Disease Gene Species

Keywords: CRISPR-dCas9; DNA methylation; chromatin architecture; histone modifications; targeted epigenome engineering; targeted gene edition; transcriptional regulation

Mesh：

Substances：
Chromatin

Year: 2017 PMID： 28985525 PMCID： PMC6205890 DOI： 10.1016/j.stem.2017.09.006

Source DB: PubMed Journal: Cell Stem Cell ISSN： 1875-9777 Impact factor: 24.633

184 in total

1. Krüppel-associated box-mediated repression of RNA polymerase II promoters is influenced by the arrangement of basal promoter elements.

Authors: G Pengue; L Lania
Journal: Proc Natl Acad Sci U S A Date: 1996-02-06 Impact factor: 11.205

2. In vivo genome editing improves muscle function in a mouse model of Duchenne muscular dystrophy.

Authors: Christopher E Nelson; Chady H Hakim; David G Ousterout; Pratiksha I Thakore; Eirik A Moreb; Ruth M Castellanos Rivera; Sarina Madhavan; Xiufang Pan; F Ann Ran; Winston X Yan; Aravind Asokan; Feng Zhang; Dongsheng Duan; Charles A Gersbach
Journal: Science Date: 2015-12-31 Impact factor: 47.728

3. Long-term dual-color tracking of genomic loci by modified sgRNAs of the CRISPR/Cas9 system.

Authors: Shipeng Shao; Weiwei Zhang; Huan Hu; Boxin Xue; Jinshan Qin; Chaoying Sun; Yuao Sun; Wensheng Wei; Yujie Sun
Journal: Nucleic Acids Res Date: 2016-02-04 Impact factor: 16.971

4. Enzymatically Generated CRISPR Libraries for Genome Labeling and Screening.

Authors: Andrew B Lane; Magdalena Strzelecka; Andreas Ettinger; Andrew W Grenfell; Torsten Wittmann; Rebecca Heald
Journal: Dev Cell Date: 2015-07-23 Impact factor: 12.270

5. Multiplexed and programmable regulation of gene networks with an integrated RNA and CRISPR/Cas toolkit in human cells.

Authors: Lior Nissim; Samuel D Perli; Alexandra Fridkin; Pablo Perez-Pinera; Timothy K Lu
Journal: Mol Cell Date: 2014-05-15 Impact factor: 17.970

6. Evaluating Synthetic Activation and Repression of Neuropsychiatric-Related Genes in hiPSC-Derived NPCs, Neurons, and Astrocytes.

Authors: Seok-Man Ho; Brigham J Hartley; Erin Flaherty; Prashanth Rajarajan; Rawan Abdelaal; Ifeanyi Obiorah; Natalie Barretto; Hamza Muhammad; Hemali P Phatnani; Schahram Akbarian; Kristen J Brennand
Journal: Stem Cell Reports Date: 2017-07-27 Impact factor: 7.765

7. Pooled CRISPR screening with single-cell transcriptome readout.

Authors: André F Rendeiro; Christian Schmidl; Paul Datlinger; Thomas Krausgruber; Peter Traxler; Johanna Klughammer; Linda C Schuster; Amelie Kuchler; Donat Alpar; Christoph Bock
Journal: Nat Methods Date: 2017-01-18 Impact factor: 28.547

8. Live cell imaging of repetitive DNA sequences via GFP-tagged polydactyl zinc finger proteins.

Authors: Beatrice I Lindhout; Paul Fransz; Federico Tessadori; Tobias Meckel; Paul J J Hooykaas; Bert J van der Zaal
Journal: Nucleic Acids Res Date: 2007-08-17 Impact factor: 16.971

9. Integrative analysis of 111 reference human epigenomes.

Authors: Anshul Kundaje; Wouter Meuleman; Jason Ernst; Misha Bilenky; Angela Yen; Alireza Heravi-Moussavi; Pouya Kheradpour; Zhizhuo Zhang; Jianrong Wang; Michael J Ziller; Viren Amin; John W Whitaker; Matthew D Schultz; Lucas D Ward; Abhishek Sarkar; Gerald Quon; Richard S Sandstrom; Matthew L Eaton; Yi-Chieh Wu; Andreas R Pfenning; Xinchen Wang; Melina Claussnitzer; Yaping Liu; Cristian Coarfa; R Alan Harris; Noam Shoresh; Charles B Epstein; Elizabeta Gjoneska; Danny Leung; Wei Xie; R David Hawkins; Ryan Lister; Chibo Hong; Philippe Gascard; Andrew J Mungall; Richard Moore; Eric Chuah; Angela Tam; Theresa K Canfield; R Scott Hansen; Rajinder Kaul; Peter J Sabo; Mukul S Bansal; Annaick Carles; Jesse R Dixon; Kai-How Farh; Soheil Feizi; Rosa Karlic; Ah-Ram Kim; Ashwinikumar Kulkarni; Daofeng Li; Rebecca Lowdon; GiNell Elliott; Tim R Mercer; Shane J Neph; Vitor Onuchic; Paz Polak; Nisha Rajagopal; Pradipta Ray; Richard C Sallari; Kyle T Siebenthall; Nicholas A Sinnott-Armstrong; Michael Stevens; Robert E Thurman; Jie Wu; Bo Zhang; Xin Zhou; Arthur E Beaudet; Laurie A Boyer; Philip L De Jager; Peggy J Farnham; Susan J Fisher; David Haussler; Steven J M Jones; Wei Li; Marco A Marra; Michael T McManus; Shamil Sunyaev; James A Thomson; Thea D Tlsty; Li-Huei Tsai; Wei Wang; Robert A Waterland; Michael Q Zhang; Lisa H Chadwick; Bradley E Bernstein; Joseph F Costello; Joseph R Ecker; Martin Hirst; Alexander Meissner; Aleksandar Milosavljevic; Bing Ren; John A Stamatoyannopoulos; Ting Wang; Manolis Kellis
Journal: Nature Date: 2015-02-19 Impact factor: 69.504

10. Waking up dormant tumor suppressor genes with zinc fingers, TALEs and the CRISPR/dCas9 system.

Authors: Benjamin Garcia-Bloj; Colette Moses; Agustin Sgro; Janice Plani-Lam; Mahira Arooj; Ciara Duffy; Shreyas Thiruvengadam; Anabel Sorolla; Rabab Rashwan; Ricardo L Mancera; Andrea Leisewitz; Theresa Swift-Scanlan; Alejandro H Corvalan; Pilar Blancafort
Journal: Oncotarget Date: 2016-09-13

78 in total

1. Robust Hi-C Maps of Enhancer-Promoter Interactions Reveal the Function of Non-coding Genome in Neural Development and Diseases.

Authors: Leina Lu; Xiaoxiao Liu; Wei-Kai Huang; Paola Giusti-Rodríguez; Jian Cui; Shanshan Zhang; Wanying Xu; Zhexing Wen; Shufeng Ma; Jonathan D Rosen; Zheng Xu; Cynthia F Bartels; Riki Kawaguchi; Ming Hu; Peter C Scacheri; Zhili Rong; Yun Li; Patrick F Sullivan; Hongjun Song; Guo-Li Ming; Yan Li; Fulai Jin
Journal: Mol Cell Date: 2020-06-26 Impact factor: 17.970

CRISPR/Cas9-Based Engineering of the Epigenome.

1. Krüppel-associated box-mediated repression of RNA polymerase II promoters is influenced by the arrangement of basal promoter elements.

2. In vivo genome editing improves muscle function in a mouse model of Duchenne muscular dystrophy.

3. Long-term dual-color tracking of genomic loci by modified sgRNAs of the CRISPR/Cas9 system.

4. Enzymatically Generated CRISPR Libraries for Genome Labeling and Screening.

5. Multiplexed and programmable regulation of gene networks with an integrated RNA and CRISPR/Cas toolkit in human cells.

6. Evaluating Synthetic Activation and Repression of Neuropsychiatric-Related Genes in hiPSC-Derived NPCs, Neurons, and Astrocytes.

7. Pooled CRISPR screening with single-cell transcriptome readout.

8. Live cell imaging of repetitive DNA sequences via GFP-tagged polydactyl zinc finger proteins.

9. Integrative analysis of 111 reference human epigenomes.

10. Waking up dormant tumor suppressor genes with zinc fingers, TALEs and the CRISPR/dCas9 system.

1. Robust Hi-C Maps of Enhancer-Promoter Interactions Reveal the Function of Non-coding Genome in Neural Development and Diseases.

Review 2. Improving Molecular Therapy in the Kidney.

3. Gene therapy for inherited arrhythmias.

Review 4. Disentangling chromatin architecture to gain insights into the etiology of brain disorders.

Review 5. Epigenetic paternal effects as costly, condition-dependent traits.

Review 6. Integrating CRISPR Engineering and hiPSC-Derived 2D Disease Modeling Systems.

Review 7. Live-Animal Epigenome Editing: Convergence of Novel Techniques.

Review 8. Constructing and Deconstructing Cancers using Human Pluripotent Stem Cells and Organoids.

9. Recent advances of genome editing and related technologies in China.

Review 10. In vivo epigenome editing and transcriptional modulation using CRISPR technology.