| Literature DB >> 26212133 |
Andrew B Lane1, Magdalena Strzelecka2, Andreas Ettinger3, Andrew W Grenfell2, Torsten Wittmann3, Rebecca Heald4.
Abstract
CRISPR-based technologies have emerged as powerful tools to alter genomes and mark chromosomal loci, but an inexpensive method for generating large numbers of RNA guides for whole genome screening and labeling is lacking. Using a method that permits library construction from any source of DNA, we generated guide libraries that label repetitive loci or a single chromosomal locus in Xenopus egg extracts and show that a complex library can target the E. coli genome at high frequency.Entities:
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Year: 2015 PMID： 26212133 PMCID： PMC4536113 DOI： 10.1016/j.devcel.2015.06.003
Source DB: PubMed Journal: Dev Cell ISSN： 1534-5807 Impact factor: 12.270