Literature DB >> 2892396

Identification of more than 500 RFLPs by screening random genomic clones.

J W Schumm1, R G Knowlton, J C Braman, D F Barker, D Botstein, G Akots, V A Brown, T C Gravius, C Helms, K Hsiao.   

Abstract

As part of our genome-mapping effort, we undertook a large-scale screening study to identify RFLPs useful as genetic markers. Some 1,664 single-copy or repeat-containing phage clones from a Charon 4A genomic library were tested for polymorphism against a panel of DNAs, from five unrelated individuals, digested with eight restriction enzymes. Approximately 30% (515) of the clones revealed polymorphism by Southern hybridization; 67 loci detected had PIC values greater than .5. Restriction enzymes MspI, TaqI, and RsaI were most efficient in detecting polymorphism within the 1-20-kb-fragment size range resolved. With only one exception each of the clones detected polymorphism originating from a single locus.

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Year:  1988        PMID： 2892396      PMCID： PMC1715337     

Source DB:  PubMed          Journal:  Am J Hum Genet        ISSN： 0002-9297            Impact factor:   11.025


  42 in total

1.  Restriction maps for twenty-one Charon vector phages.

Authors:  J R de Wet; D L Daniels; J L Schroeder; B G Williams; K Denniston-Thompson; D D Moore; F R Blattner
Journal:  J Virol       Date:  1980-01       Impact factor: 5.103

2.  Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors:  P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal:  J Mol Biol       Date:  1977-06-15       Impact factor: 5.469

3.  Construction of a map of the short arm of human chromosome 6.

Authors:  R Leach; R DeMars; S Hasstedt; R White
Journal:  Proc Natl Acad Sci U S A       Date:  1986-06       Impact factor: 11.205

4.  Screening lambdagt recombinant clones by hybridization to single plaques in situ.

Authors:  W D Benton; R W Davis
Journal:  Science       Date:  1977-04-08       Impact factor: 47.728

5.  Estimation of the recombination fraction in human pedigrees: efficient computation of the likelihood for human linkage studies.

Authors:  J Ott
Journal:  Am J Hum Genet       Date:  1974-09       Impact factor: 11.025

6.  Report of the Committee on Human Gene Mapping by Recombinant DNA Techniques.

Authors:  H F Willard; M H Skolnick; P L Pearson; J L Mandel
Journal:  Cytogenet Cell Genet       Date:  1985

7.  Highly polymorphic RFLP probes as diagnostic tools.

Authors:  H Donis-Keller; D F Barker; R G Knowlton; J W Schumm; J C Braman; P Green
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1986

8.  Evolution of sickle variant gene.

Authors:  E Solomon; W F Bodmer
Journal:  Lancet       Date:  1979-04-28       Impact factor: 79.321

9.  The isolation and characterization of linked delta- and beta-globin genes from a cloned library of human DNA.

Authors:  R M Lawn; E F Fritsch; R C Parker; G Blake; T Maniatis
Journal:  Cell       Date:  1978-12       Impact factor: 41.582

10.  DNA sequence variants in the G gamma-, A gamma-, delta- and beta-globin genes of man.

Authors:  A J Jeffreys
Journal:  Cell       Date:  1979-09       Impact factor: 41.582
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  16 in total

1.  A new TaqI allele detected by the CRI-R227 (D4S101) probe in Pima Indians.

Authors:  M G Choi; M Prochazka; P Thuillez; S Lillioja
Journal:  Nucleic Acids Res       Date:  1992-03-11       Impact factor: 16.971

2.  Genetic linkage map of 46 DNA markers on human chromosome 16.

Authors:  T P Keith; P Green; S T Reeders; V A Brown; P Phipps; A Bricker; K Falls; K S Rediker; J A Powers; C Hogan
Journal:  Proc Natl Acad Sci U S A       Date:  1990-08       Impact factor: 11.205

3.  Identification and characterization of 23 RFLP loci by screening random cosmid genomic clones.

Authors:  D W Bowden; H Müller-Kahle; T C Gravius; C Helms; D Watt-Morgan; P Green; H Donis-Keller
Journal:  Am J Hum Genet       Date:  1989-05       Impact factor: 11.025

4.  Localization of preferential sites of rearrangement within the BCR gene in Philadelphia chromosome-positive acute lymphoblastic leukemia.

Authors:  C T Denny; N P Shah; S Ogden; C Willman; T McConnell; W Crist; A Carroll; O N Witte
Journal:  Proc Natl Acad Sci U S A       Date:  1989-06       Impact factor: 11.205

5.  Detection of DNA sequence polymorphisms by enzymatic amplification and direct genomic sequencing.

Authors:  D W Yandell; T P Dryja
Journal:  Am J Hum Genet       Date:  1989-10       Impact factor: 11.025

6.  Polymers of random short oligonucleotides detect polymorphic loci in the human genome.

Authors:  G Vergnaud
Journal:  Nucleic Acids Res       Date:  1989-10-11       Impact factor: 16.971

7.  M13 repeat probe detects DNA minisatellite-like sequences in gymnosperms and angiosperms.

Authors:  S H Rogstad; J C Patton; B A Schaal
Journal:  Proc Natl Acad Sci U S A       Date:  1988-12       Impact factor: 11.205

8.  Abundant class of human DNA polymorphisms which can be typed using the polymerase chain reaction.

Authors:  J L Weber; P E May
Journal:  Am J Hum Genet       Date:  1989-03       Impact factor: 11.025

9.  An extremely polymorphic locus on the short arm of the human X chromosome with homology to the long arm of the Y chromosome.

Authors:  R G Knowlton; C A Nelson; V A Brown; D C Page; H Donis-Keller
Journal:  Nucleic Acids Res       Date:  1989-01-11       Impact factor: 16.971

10.  Molecular and genetic characterization and physical mapping of 11 new markers detecting multiallele restriction fragment length polymorphisms on the short arm of human chromosome 3.

Authors:  F Latif; W S Modi; F M Duh; L Schmidt; H Li; L Geil; M L Orcutt; A Heppell-Parton; P H Rabbitts; W M Linehan
Journal:  Hum Genet       Date:  1992 Sep-Oct       Impact factor: 4.132
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