Literature DB >> 2565079

Identification and characterization of 23 RFLP loci by screening random cosmid genomic clones.

D W Bowden1, H Müller-Kahle, T C Gravius, C Helms, D Watt-Morgan, P Green, H Donis-Keller.   

Abstract

As part of our search for polymorphic DNA probes, we have screened cosmids from a human genomic DNA library for their ability to reveal RFLPs. A total of 101 randomly isolated cosmid clones were tested in Southern hybridizations for polymorphic band patterns. Fifty-four of these clones revealed RFLPs with one or more of nine restriction enzymes. Twenty-three of these clones have been further characterized and assigned to 10 different chromosomes by linkage analysis or by hybridization to panels of human-hamster hybrid cell lines. Fifteen of the probes have heterozygosities greater than or equal to .5. The relative efficiency of RsaI and PstI restriction enzymes in detecting polymorphism was different from results obtained with libraries constructed in bacteriophage vectors. Screening randomly selected cosmid probes is an efficient method for detecting RFLPs.

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Year:  1989        PMID： 2565079      PMCID： PMC1715641     

Source DB:  PubMed          Journal:  Am J Hum Genet        ISSN： 0002-9297            Impact factor:   11.025


  13 in total

1.  Variable number of tandem repeat (VNTR) markers for human gene mapping.

Authors:  Y Nakamura; M Leppert; P O'Connell; R Wolff; T Holm; M Culver; C Martin; E Fujimoto; M Hoff; E Kumlin
Journal:  Science       Date:  1987-03-27       Impact factor: 47.728

2.  A genetic linkage map of the human genome.

Authors:  H Donis-Keller; P Green; C Helms; S Cartinhour; B Weiffenbach; K Stephens; T P Keith; D W Bowden; D R Smith; E S Lander
Journal:  Cell       Date:  1987-10-23       Impact factor: 41.582

3.  Occurrence of a transposition from the X-chromosome long arm to the Y-chromosome short arm during human evolution.

Authors:  D C Page; M E Harper; J Love; D Botstein
Journal:  Nature       Date:  1984 Sep 13-19       Impact factor: 49.962

4.  Studies on locus expansion, library representation, and chromosome walking using an efficient method to screen cosmid libraries.

Authors:  D W Bowden; H Müller-Kahle; T R Fulton; T C Gravius; D F Barker; H Donis-Keller
Journal:  Gene       Date:  1988-11-30       Impact factor: 3.688

5.  Rapid and efficient cosmid cloning.

Authors:  D Ish-Horowicz; J F Burke
Journal:  Nucleic Acids Res       Date:  1981-07-10       Impact factor: 16.971

6.  Genetic mapping of the human X chromosome by using restriction fragment length polymorphisms.

Authors:  D Drayna; K Davies; D Hartley; J L Mandel; G Camerino; R Williamson; R White
Journal:  Proc Natl Acad Sci U S A       Date:  1984-05       Impact factor: 11.205

7.  Double cos site vectors: simplified cosmid cloning.

Authors:  P F Bates; R A Swift
Journal:  Gene       Date:  1983-12       Impact factor: 3.688

8.  Construction of multilocus genetic linkage maps in humans.

Authors:  E S Lander; P Green
Journal:  Proc Natl Acad Sci U S A       Date:  1987-04       Impact factor: 11.205

9.  Restriction sites containing CpG show a higher frequency of polymorphism in human DNA.

Authors:  D Barker; M Schafer; R White
Journal:  Cell       Date:  1984-01       Impact factor: 41.582

10.  A highly polymorphic locus in human DNA revealed by cosmid-derived probes.

Authors:  M Litt; R L White
Journal:  Proc Natl Acad Sci U S A       Date:  1985-09       Impact factor: 11.205
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  4 in total

1.  Framework multipoint map of the long arm of human chromosome 4 and telomeric localization of the gene for FSHD.

Authors:  B Weiffenbach; R G Bagley; K Falls; J Dubois; C Hyser; D Storvick; P Schultz; J R Mendell; E C Milner; S J Jacobsen
Journal:  Mamm Genome       Date:  1992       Impact factor: 2.957

2.  Cosmid probe CRI-C47 (D4S117) maps to 4p16.1.

Authors:  B Weiffenbach; M Altherr; J J Wasmuth
Journal:  Nucleic Acids Res       Date:  1991-04-11       Impact factor: 16.971

3.  High-resolution genetic mapping of the hypodactyly (Hd) locus on mouse chromosome 6.

Authors:  J W Innis; K Kazen-Gillespie; L C Post; J McGorman
Journal:  Mamm Genome       Date:  1996-01       Impact factor: 2.957

4.  A genetic map of chromosome 20q12-q13.1: multiple highly polymorphic microsatellite and RFLP markers linked to the maturity-onset diabetes of the young (MODY) locus.

Authors:  C B Rothschild; G Akots; R Hayworth; M J Pettenati; P N Rao; P Wood; F M Stolz; I Hansmann; K Serino; T P Keith
Journal:  Am J Hum Genet       Date:  1993-01       Impact factor: 11.025
  4 in total

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