| Literature DB >> 28892089 |
Yuta Nihongaki1, Yuichi Furuhata2, Takahiro Otabe1, Saki Hasegawa1, Keitaro Yoshimoto1, Moritoshi Sato1.
Abstract
Our improved CRISPR-Cas9-based photoactivatable transcription systems, CPTS2.0 and Split-CPTS2.0, enable high blue-light-inducible activation of endogenous target genes in various human cell lines. We achieved reversible activation of target genes with CPTS2.0 and induced neuronal differentiation in induced pluripotent stem cells (iPSCs) by upregulating NEUROD1 with Split-CPTS2.0.Entities:
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Year: 2017 PMID： 28892089 DOI： 10.1038/nmeth.4430
Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547