Literature DB >> 28736667

Changes in optical properties of electroporated cells as revealed by digital holographic microscopy.

Violeta L Calin1, Mona Mihailescu2, Nicolae Mihale2, Alexandra V Baluta3, Eugenia Kovacs1, Tudor Savopol1, Mihaela G Moisescu1.   

Abstract

Changes in optical and shape-related characteristics of B16F10 cells after electroporation were investigated using digital holographic microscopy (DHM). Bipolar rectangular pulses specific for electrochemotherapy were used. Electroporation was performed in an "off-axis" DHM set-up without using exogenous markers. Two types of cell parameters were monitored seconds and minutes after pulse train application: parameters addressing a specifically defined area of the cell (refractive index and cell height) and global cell parameters (projected area, optical phase shift profile and dry mass). The biphasic behavior of cellular parameters was explained by water and mannitol dynamics through the electropermeabilized cell membrane.

Entities:  

Keywords:  (090.1995) Digital holography; (170.0170) Medical optics and biotechnology; (170.1530) Cell analysis

Year:  2017        PMID： 28736667      PMCID： PMC5516823          DOI： 10.1364/BOE.8.002222

Source DB:  PubMed          Journal:  Biomed Opt Express        ISSN： 2156-7085            Impact factor:   3.732


  42 in total

1.  Manipulation of cell volume and membrane pore comparison following single cell permeabilization with 60- and 600-ns electric pulses.

Authors:  Olena M Nesin; Olga N Pakhomova; Shu Xiao; Andrei G Pakhomov
Journal:  Biochim Biophys Acta       Date:  2010-12-20

2.  Electropermeabilization, a physical method for the delivery of therapeutic molecules into cells.

Authors:  Marie-Pierre Rols
Journal:  Biochim Biophys Acta       Date:  2006-01-30

3.  Tomographic phase microscopy.

Authors:  Wonshik Choi; Christopher Fang-Yen; Kamran Badizadegan; Seungeun Oh; Niyom Lue; Ramachandra R Dasari; Michael S Feld
Journal:  Nat Methods       Date:  2007-08-12       Impact factor: 28.547

4.  Measurement of the integral refractive index and dynamic cell morphometry of living cells with digital holographic microscopy.

Authors:  Benjamin Rappaz; Pierre Marquet; Etienne Cuche; Yves Emery; Christian Depeursinge; Pierre Magistretti
Journal:  Opt Express       Date:  2005-11-14       Impact factor: 3.894

5.  Non-invasive, label-free cell counting and quantitative analysis of adherent cells using digital holography.

Authors:  A Mölder; M Sebesta; M Gustafsson; L Gisselson; A Gjörloff Wingren; K Alm
Journal:  J Microsc       Date:  2008-11       Impact factor: 1.758

Review 6.  Electroporation for drug and gene delivery in the clinic: doctors go electric.

Authors:  Julie Gehl
Journal:  Methods Mol Biol       Date:  2008

Review 7.  Mechanisms of transfer of bioactive molecules through the cell membrane by electroporation.

Authors:  Mindaugas S Venslauskas; Saulius Šatkauskas
Journal:  Eur Biophys J       Date:  2015-05-05       Impact factor: 1.733

8.  Comparison of the effects of the repetition rate between microsecond and nanosecond pulses: electropermeabilization-induced electro-desensitization?

Authors:  A Silve; A Guimerà Brunet; B Al-Sakere; A Ivorra; L M Mir
Journal:  Biochim Biophys Acta       Date:  2014-02-28

Review 9.  Electroporation-based gene therapy: recent evolution in the mechanism description and technology developments.

Authors:  Lluis M Mir
Journal:  Methods Mol Biol       Date:  2014

10.  Membrane disorder and phospholipid scrambling in electropermeabilized and viable cells.

Authors:  Jean-Michel Escoffre; Elisabeth Bellard; Cécile Faurie; Sarra C Sébaï; Muriel Golzio; Justin Teissié; Marie-Pierre Rols
Journal:  Biochim Biophys Acta       Date:  2014-02-26
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  3 in total

1.  Quantitative observations on cytoskeleton changes of osteocytes at different cell parts using digital holographic microscopy.

Authors:  Runyu Cao; Wen Xiao; Xintong Wu; Lianwen Sun; Feng Pan
Journal:  Biomed Opt Express       Date:  2017-12-05       Impact factor: 3.732

2.  Morphological changes in the ovarian carcinoma cells of Wistar rats induced by chemotherapy with cisplatin and dioxadet.

Authors:  A A Zhikhoreva; A V Belashov; V G Bespalov; A L Semenov; I V Semenova; G V Tochilnikov; N T Zhilinskaya; O S Vasyutinskii
Journal:  Biomed Opt Express       Date:  2018-10-29       Impact factor: 3.732

3.  Evaluation of the metastatic potential of malignant cells by image processing of digital holographic microscopy data.

Authors:  Violeta L Calin; Mona Mihailescu; Eugen I Scarlat; Alexandra V Baluta; Daniel Calin; Eugenia Kovacs; Tudor Savopol; Mihaela G Moisescu
Journal:  FEBS Open Bio       Date:  2017-09-02       Impact factor: 2.693
  3 in total

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