| Literature DB >> 17694065 |
Wonshik Choi1, Christopher Fang-Yen, Kamran Badizadegan, Seungeun Oh, Niyom Lue, Ramachandra R Dasari, Michael S Feld.
Abstract
We report a technique for quantitative three-dimensional (3D) mapping of refractive index in live cells and tissues using a phase-shifting laser interferometric microscope with variable illumination angle. We demonstrate tomographic imaging of cells and multicellular organisms, and time-dependent changes in cell structure. Our results will permit quantitative characterization of specimen-induced aberrations in high-resolution microscopy and have multiple applications in tissue light scattering.Mesh:
Year: 2007 PMID: 17694065 DOI: 10.1038/nmeth1078
Source DB: PubMed Journal: Nat Methods ISSN: 1548-7091 Impact factor: 28.547