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Literature DB >> 28625887

Time-resolved cryo-electron microscopy: Recent progress.

Abstract

Time-resolved cryo-electron microscopy (cryo-EM) combines the known advantages of single-particle cryo-EM in visualizing molecular structure with the ability to dissect the time progress of a reaction between molecules in vitro. Here some of the recent progress of this methodology and its first biological applications are outlined.

Entities: Chemical

Keywords: Cryo-EM; Microfluidics; RRF; Ribosome recycling; Short-lived states

Mesh：

Year: 2017 PMID： 28625887 PMCID： PMC5732889 DOI： 10.1016/j.jsb.2017.06.005

Source DB: PubMed Journal: J Struct Biol ISSN： 1047-8477 Impact factor: 2.867

14 in total

1. A second generation apparatus for time-resolved electron cryo-microscopy using stepper motors and electrospray.

Authors: H D White; K Thirumurugan; M L Walker; J Trinick
Journal: J Struct Biol Date: 2003 Oct-Nov Impact factor: 2.867

2. Ribosome dynamics and tRNA movement by time-resolved electron cryomicroscopy.

Authors: Niels Fischer; Andrey L Konevega; Wolfgang Wintermeyer; Marina V Rodnina; Holger Stark
Journal: Nature Date: 2010-07-15 Impact factor: 49.962

Review 3. Two promising future developments of cryo-EM: capturing short-lived states and mapping a continuum of states of a macromolecule.

Authors: Bo Chen; Joachim Frank
Journal: Microscopy (Oxf) Date: 2015-10-31 Impact factor: 1.571

4. Splitting of the posttermination ribosome into subunits by the concerted action of RRF and EF-G.

Authors: Andrey V Zavialov; Vasili V Hauryliuk; Måns Ehrenberg
Journal: Mol Cell Date: 2005-06-10 Impact factor: 17.970

5. Initial bridges between two ribosomal subunits are formed within 9.4 milliseconds, as studied by time-resolved cryo-EM.

Authors: Tanvir R Shaikh; Aymen S Yassin; Zonghuan Lu; David Barnard; Xing Meng; Toh-Ming Lu; Terence Wagenknecht; Rajendra K Agrawal
Journal: Proc Natl Acad Sci U S A Date: 2014-06-23 Impact factor: 11.205

6. Analysis of transient structures by cryo-microscopy combined with rapid mixing of spray droplets.

Authors: J Berriman; N Unwin
Journal: Ultramicroscopy Date: 1994-12 Impact factor: 2.689

7. Modular Assembly of the Bacterial Large Ribosomal Subunit.

Authors: Joseph H Davis; Yong Zi Tan; Bridget Carragher; Clinton S Potter; Dmitry Lyumkis; James R Williamson
Journal: Cell Date: 2016-12-01 Impact factor: 41.582

8. Monolithic microfluidic mixing-spraying devices for time-resolved cryo-electron microscopy.

Authors: Zonghuan Lu; Tanvir R Shaikh; David Barnard; Xing Meng; Hisham Mohamed; Aymen Yassin; Carmen A Mannella; Rajendra K Agrawal; Toh-Ming Lu; Terence Wagenknecht
Journal: J Struct Biol Date: 2009-08-14 Impact factor: 2.867

9. A Fast and Effective Microfluidic Spraying-Plunging Method for High-Resolution Single-Particle Cryo-EM.

Authors: Xiangsong Feng; Ziao Fu; Sandip Kaledhonkar; Yuan Jia; Binita Shah; Amy Jin; Zheng Liu; Ming Sun; Bo Chen; Robert A Grassucci; Yukun Ren; Hongyuan Jiang; Joachim Frank; Qiao Lin
Journal: Structure Date: 2017-03-09 Impact factor: 5.006

10. RELION: implementation of a Bayesian approach to cryo-EM structure determination.

Authors: Sjors H W Scheres
Journal: J Struct Biol Date: 2012-09-19 Impact factor: 2.867

29 in total

Review 1. Relating Structure and Dynamics in RNA Biology.

Authors: Kevin P Larsen; Junhong Choi; Arjun Prabhakar; Elisabetta Viani Puglisi; Joseph D Puglisi
Journal: Cold Spring Harb Perspect Biol Date: 2019-07-01 Impact factor: 10.005

Review 2. Challenges and opportunities in cryo-EM single-particle analysis.

Authors: Dmitry Lyumkis
Journal: J Biol Chem Date: 2019-02-25 Impact factor: 5.157

3. Routine single particle CryoEM sample and grid characterization by tomography.

Authors: Venkata P Dandey; Hui Wei; Alex J Noble; Julia Brasch; Jillian Chase; Priyamvada Acharya; Yong Zi Tan; Zhening Zhang; Laura Y Kim; Giovanna Scapin; Micah Rapp; Edward T Eng; William J Rice; Anchi Cheng; Carl J Negro; Lawrence Shapiro; Peter D Kwong; David Jeruzalmi; Amedee des Georges; Clinton S Potter; Bridget Carragher
Journal: Elife Date: 2018-05-29 Impact factor: 8.140

Time-resolved cryo-electron microscopy: Recent progress.

1. A second generation apparatus for time-resolved electron cryo-microscopy using stepper motors and electrospray.

2. Ribosome dynamics and tRNA movement by time-resolved electron cryomicroscopy.

Review 3. Two promising future developments of cryo-EM: capturing short-lived states and mapping a continuum of states of a macromolecule.

4. Splitting of the posttermination ribosome into subunits by the concerted action of RRF and EF-G.

5. Initial bridges between two ribosomal subunits are formed within 9.4 milliseconds, as studied by time-resolved cryo-EM.

6. Analysis of transient structures by cryo-microscopy combined with rapid mixing of spray droplets.

7. Modular Assembly of the Bacterial Large Ribosomal Subunit.

8. Monolithic microfluidic mixing-spraying devices for time-resolved cryo-electron microscopy.

9. A Fast and Effective Microfluidic Spraying-Plunging Method for High-Resolution Single-Particle Cryo-EM.

10. RELION: implementation of a Bayesian approach to cryo-EM structure determination.

Review 1. Relating Structure and Dynamics in RNA Biology.

Review 2. Challenges and opportunities in cryo-EM single-particle analysis.

3. Routine single particle CryoEM sample and grid characterization by tomography.

4. X-rays in the Cryo-Electron Microscopy Era: Structural Biology's Dynamic Future.

Review 5. Determining translocation orientations of nucleic acid helicases.

6. Resolving Dynamics in the Ensemble: Finding Paths through Intermediate States and Disordered Protein Structures.

7. CryoEM structural exploration of catalytically active enzyme pyruvate carboxylase.

Review 8. Unconventional Imaging Methods to Capture Transient Structures during Actomyosin Interaction.

Review 9. Advances in methods for atomic resolution macromolecular structure determination.

10. Modular microfluidics enables kinetic insight from time-resolved cryo-EM.