Literature DB >> 28603751

Production of Guide RNAs in vitro and in vivo for CRISPR Using Ribozymes and RNA Polymerase II Promoters.

Tao Zhang1, Yangbin Gao2, Rongchen Wang1, Yunde Zhao1,2.   

Abstract

CRISPR/Cas9-mediated genome editing relies on a guide RNA (gRNA) molecule to generate sequence-specific DNA cleavage, which is a prerequisite for gene editing. Here we establish a method that enables production of gRNAs from any promoters, in any organisms, and in vitro (Gao and Zhao, 2014). This method also makes it feasible to conduct tissue/cell specific gene editing.

Entities:  

Keywords:  CRISPR; Genome editing; RNA polymerase II promoter; RNA transcription; Ribozyme

Year:  2017        PMID： 28603751      PMCID： PMC5463609          DOI： 10.21769/BioProtoc.2148

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  9 in total

1.  Enzymatic assembly of DNA molecules up to several hundred kilobases.

Authors:  Daniel G Gibson; Lei Young; Ray-Yuan Chuang; J Craig Venter; Clyde A Hutchison; Hamilton O Smith
Journal:  Nat Methods       Date:  2009-04-12       Impact factor: 28.547

2.  Self-processing of ribozyme-flanked RNAs into guide RNAs in vitro and in vivo for CRISPR-mediated genome editing.

Authors:  Yangbin Gao; Yunde Zhao
Journal:  J Integr Plant Biol       Date:  2014-03-06       Impact factor: 7.061

3.  Auxin binding protein 1 (ABP1) is not required for either auxin signaling or Arabidopsis development.

Authors:  Yangbin Gao; Yi Zhang; Da Zhang; Xinhua Dai; Mark Estelle; Yunde Zhao
Journal:  Proc Natl Acad Sci U S A       Date:  2015-02-02       Impact factor: 11.205

4.  Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana.

Authors:  S J Clough; A F Bent
Journal:  Plant J       Date:  1998-12       Impact factor: 6.417

5.  Multiplex genome engineering using CRISPR/Cas systems.

Authors:  Le Cong; F Ann Ran; David Cox; Shuailiang Lin; Robert Barretto; Naomi Habib; Patrick D Hsu; Xuebing Wu; Wenyan Jiang; Luciano A Marraffini; Feng Zhang
Journal:  Science       Date:  2013-01-03       Impact factor: 47.728

6.  RNA-guided human genome engineering via Cas9.

Authors:  Prashant Mali; Luhan Yang; Kevin M Esvelt; John Aach; Marc Guell; James E DiCarlo; Julie E Norville; George M Church
Journal:  Science       Date:  2013-01-03       Impact factor: 47.728

7.  An Effective Strategy for Reliably Isolating Heritable and Cas9-Free Arabidopsis Mutants Generated by CRISPR/Cas9-Mediated Genome Editing.

Authors:  Xiuhua Gao; Jilin Chen; Xinhua Dai; Da Zhang; Yunde Zhao
Journal:  Plant Physiol       Date:  2016-05-15       Impact factor: 8.340

8.  Multiplexed and programmable regulation of gene networks with an integrated RNA and CRISPR/Cas toolkit in human cells.

Authors:  Lior Nissim; Samuel D Perli; Alexandra Fridkin; Pablo Perez-Pinera; Timothy K Lu
Journal:  Mol Cell       Date:  2014-05-15       Impact factor: 17.970

9.  Development of a mono-promoter-driven CRISPR/Cas9 system in mammalian cells.

Authors:  Shin Yoshioka; Wataru Fujii; Tetsuhiro Ogawa; Koji Sugiura; Kunihiko Naito
Journal:  Sci Rep       Date:  2015-12-16       Impact factor: 4.379
  9 in total
  13 in total

1.  Precise gene replacement in rice by RNA transcript-templated homologous recombination.

Authors:  Shaoya Li; Jingying Li; Yubing He; Meilian Xu; Jiahui Zhang; Wenming Du; Yunde Zhao; Lanqin Xia
Journal:  Nat Biotechnol       Date:  2019-03-18       Impact factor: 54.908

Review 2.  CRISPR-based genome editing in wheat: a comprehensive review and future prospects.

Authors:  Rakesh Kumar; Amandeep Kaur; Ankita Pandey; H M Mamrutha; G P Singh
Journal:  Mol Biol Rep       Date:  2019-04-02       Impact factor: 2.316

3.  Reducing resistance allele formation in CRISPR gene drive.

Authors:  Jackson Champer; Jingxian Liu; Suh Yeon Oh; Riona Reeves; Anisha Luthra; Nathan Oakes; Andrew G Clark; Philipp W Messer
Journal:  Proc Natl Acad Sci U S A       Date:  2018-05-07       Impact factor: 11.205

4.  CRISPR Gene Drive Efficiency and Resistance Rate Is Highly Heritable with No Common Genetic Loci of Large Effect.

Authors:  Jackson Champer; Zhaoxin Wen; Anisha Luthra; Riona Reeves; Joan Chung; Chen Liu; Yoo Lim Lee; Jingxian Liu; Emily Yang; Philipp W Messer; Andrew G Clark
Journal:  Genetics       Date:  2019-03-27       Impact factor: 4.562

5.  PINOID Is Required for Formation of the Stigma and Style in Rice.

Authors:  Yubing He; Lang Yan; Chennan Ge; Xue-Feng Yao; Xiang Han; Rongchen Wang; Lizhong Xiong; Liwen Jiang; Chun-Ming Liu; Yunde Zhao
Journal:  Plant Physiol       Date:  2019-03-27       Impact factor: 8.340

6.  Preparation of Cas9 Ribonucleoproteins for Genome Editing.

Authors:  Sheng-Wei Lin; Viet Quoc Nguyen; Steven Lin
Journal:  Bio Protoc       Date:  2022-05-20

Review 7.  In vivo epigenome editing and transcriptional modulation using CRISPR technology.

Authors:  Cia-Hin Lau; Yousin Suh
Journal:  Transgenic Res       Date:  2018-10-04       Impact factor: 2.788

Review 8.  New and emerging uses of CRISPR/Cas9 to genetically manipulate apicomplexan parasites.

Authors:  Manlio Di Cristina; Vern B Carruthers
Journal:  Parasitology       Date:  2018-02-21       Impact factor: 3.234

9.  A Drosophila CRISPR/Cas9 Toolkit for Conditionally Manipulating Gene Expression in the Prothoracic Gland as a Test Case for Polytene Tissues.

Authors:  Nhan Huynh; Jie Zeng; Wen Liu; Kirst King-Jones
Journal:  G3 (Bethesda)       Date:  2018-11-06       Impact factor: 3.154

10.  Efficient genome editing using tRNA promoter-driven CRISPR/Cas9 gRNA in Aspergillus niger.

Authors:  Letian Song; Jean-Paul Ouedraogo; Magdalena Kolbusz; Thi Truc Minh Nguyen; Adrian Tsang
Journal:  PLoS One       Date:  2018-08-24       Impact factor: 3.240
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