Literature DB >> 28597155

Discriminatory power of rbcL barcode locus for authentication of some of United Arab Emirates (UAE) native plants.

Lina Maloukh1, Alagappan Kumarappan1, Mohammad Jarrar1, Jawad Salehi1, Houssam El-Wakil1, T V Rajya Lakshmi2.   

Abstract

DNA barcoding of United Arab Emirates (UAE) native plants is of high practical and scientific value as the plants adapt to very harsh environmental conditions that challenge their identification. Fifty-one plant species belonged to 22 families, 2 monocots, and 20 eudicots; a maximum number of species being legumes and grasses were collected. To authenticate the morphological identification of the wild plant taxa, rbcL and matK regions were used in the study. The primer universality and discriminatory power of rbcL is 100%, while it is 35% for matK locus for these plant species. The sequences were submitted to GenBank; accession numbers were obtained for all the rbcL sequences and for 6 of matK sequences. We suggest rbcL as a promising barcode locus for the tested group of 51 plants. In the present study, an inexpensive, simple method of identification of rare desert plant taxa through rbcL barcode is being reported.

Entities:  

Keywords:  Barcode; Eudicots; Monocots; Native plants; United Arab Emirates; matK; rbcL

Year:  2017        PMID: 28597155      PMCID: PMC5465043          DOI: 10.1007/s13205-017-0746-1

Source DB:  PubMed          Journal:  3 Biotech        ISSN: 2190-5738            Impact factor:   2.406


  27 in total

1.  DNA barcoding methods for land plants.

Authors:  Aron J Fazekas; Maria L Kuzmina; Steven G Newmaster; Peter M Hollingsworth
Journal:  Methods Mol Biol       Date:  2012

2.  Quantification of the detrimental effect of a single primer-template mismatch by real-time PCR using the 16S rRNA gene as an example.

Authors:  D Bru; F Martin-Laurent; L Philippot
Journal:  Appl Environ Microbiol       Date:  2008-01-11       Impact factor: 4.792

Review 3.  20 years since the introduction of DNA barcoding: from theory to application.

Authors:  Živa Fišer Pečnikar; Elena V Buzan
Journal:  J Appl Genet       Date:  2013-11-08       Impact factor: 3.240

4.  Ecology. Barcoding of plants and fungi.

Authors:  Mark W Chase; Michael F Fay
Journal:  Science       Date:  2009-07-30       Impact factor: 47.728

5.  A DNA barcode for land plants.

Authors: 
Journal:  Proc Natl Acad Sci U S A       Date:  2009-07-30       Impact factor: 11.205

6.  Selecting barcoding loci for plants: evaluation of seven candidate loci with species-level sampling in three divergent groups of land plants.

Authors:  Michelle L Hollingsworth; Alex Andra Clark; Laura L Forrest; James Richardson; R Toby Pennington; David G Long; Robyn Cowan; Mark W Chase; Myriam Gaudeul; Peter M Hollingsworth
Journal:  Mol Ecol Resour       Date:  2009-01-31       Impact factor: 7.090

7.  The neighbor-joining method: a new method for reconstructing phylogenetic trees.

Authors:  N Saitou; M Nei
Journal:  Mol Biol Evol       Date:  1987-07       Impact factor: 16.240

8.  Bryophyte DNA sequences from faeces of an arctic herbivore, barnacle goose (Branta leucopsis).

Authors:  M Stech; E Kolvoort; M J J E Loonen; K Vrieling; J D Kruijer
Journal:  Mol Ecol Resour       Date:  2010-11-10       Impact factor: 7.090

9.  Detecting ingested plant DNA in soil-living insect larvae.

Authors:  Karin Staudacher; Corinna Wallinger; Nikolaus Schallhart; Michael Traugott
Journal:  Soil Biol Biochem       Date:  2011-02       Impact factor: 7.609

10.  DNA barcoding in the cycadales: testing the potential of proposed barcoding markers for species identification of cycads.

Authors:  Chodon Sass; Damon P Little; Dennis Wm Stevenson; Chelsea D Specht
Journal:  PLoS One       Date:  2007-11-07       Impact factor: 3.240

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  7 in total

1.  Assessment of three plastid DNA barcode markers for identification of Clinacanthus nutans (Acanthaceae).

Authors:  Noor Zafirah Ismail; Hasni Arsad; Mohammed Razip Samian; Mohammad Razak Hamdan; Ahmad Sofiman Othman
Journal:  3 Biotech       Date:  2018-01-11       Impact factor: 2.406

Review 2.  The Promise of Molecular and Genomic Techniques for Biodiversity Research and DNA Barcoding of the Arabian Peninsula Flora.

Authors:  Kareem A Mosa; Sanjay Gairola; Rahul Jamdade; Ali El-Keblawy; Khawla Ibrahim Al Shaer; Eman Khalid Al Harthi; Hatem A Shabana; Tamer Mahmoud
Journal:  Front Plant Sci       Date:  2019-01-21       Impact factor: 5.753

3.  Evaluation of Arabian Vascular Plant Barcodes (rbcL and matK): Precision of Unsupervised and Supervised Learning Methods towards Accurate Identification.

Authors:  Rahul Jamdade; Maulik Upadhyay; Khawla Al Shaer; Eman Al Harthi; Mariam Al Sallani; Mariam Al Jasmi; Asma Al Ketbi
Journal:  Plants (Basel)       Date:  2021-12-13

4.  Testing the effectiveness of rbcLa DNA-barcoding for species discrimination in tropical montane cloud forest vascular plants (Oaxaca, Mexico) using BLAST, genetic distance, and tree-based methods.

Authors:  Sonia Trujillo-Argueta; Rafael F Del Castillo; Abril Velasco-Murguía
Journal:  PeerJ       Date:  2022-08-16       Impact factor: 3.061

5.  Assessing Phenotypic Variability in Some Eastern European Insular Populations of the Climatic Relict Ilex aquifolium L.

Authors:  Ciprian Valentin Mihali; Constantin Marian Petrescu; Calin Flavius Ciolacu-Ladasiu; Endre Mathe; Cristina Popescu; Viviane Bota; Alexandru Eugeniu Mizeranschi; Daniela Elena Ilie; Radu Ionel Neamț; Violeta Turcus
Journal:  Plants (Basel)       Date:  2022-08-03

6.  DNA barcodes for delineating Clerodendrum species of North East India.

Authors:  Barbi Gogoi; S B Wann; S P Saikia
Journal:  Sci Rep       Date:  2020-08-10       Impact factor: 4.379

7.  Genetic Characterization of an Endangered Chilean Endemic Species, Prosopis burkartii Muñoz, Reveals its Hybrids Parentage.

Authors:  Roberto Contreras; Liesbeth van den Brink; Boris Burgos; Marlene González; Sandra Gacitúa
Journal:  Plants (Basel)       Date:  2020-06-12
  7 in total

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