| Literature DB >> 28481340 |
Stephen J Mondo1, Richard O Dannebaum1, Rita C Kuo1, Katherine B Louie1, Adam J Bewick2, Kurt LaButti1, Sajeet Haridas1, Alan Kuo1, Asaf Salamov1, Steven R Ahrendt1,3, Rebecca Lau1, Benjamin P Bowen1, Anna Lipzen1, William Sullivan1, Bill B Andreopoulos1, Alicia Clum1, Erika Lindquist1, Christopher Daum1, Trent R Northen1, Govindarajan Kunde-Ramamoorthy1, Robert J Schmitz2, Andrii Gryganskyi4, David Culley5, Jon Magnuson5, Timothy Y James6, Michelle A O'Malley7, Jason E Stajich8, Joseph W Spatafora9, Axel Visel1,10, Igor V Grigoriev1,3.
Abstract
N6-methyldeoxyadenine (6mA) is a noncanonical DNA base modification present at low levels in plant and animal genomes, but its prevalence and association with genome function in other eukaryotic lineages remains poorly understood. Here we report that abundant 6mA is associated with transcriptionally active genes in early-diverging fungal lineages. Using single-molecule long-read sequencing of 16 diverse fungal genomes, we observed that up to 2.8% of all adenines were methylated in early-diverging fungi, far exceeding levels observed in other eukaryotes and more derived fungi. 6mA occurred symmetrically at ApT dinucleotides and was concentrated in dense methylated adenine clusters surrounding the transcriptional start sites of expressed genes; its distribution was inversely correlated with that of 5-methylcytosine. Our results show a striking contrast in the genomic distributions of 6mA and 5-methylcytosine and reinforce a distinct role for 6mA as a gene-expression-associated epigenomic mark in eukaryotes.Entities:
Mesh：
Substances：
Year: 2017 PMID： 28481340 DOI： 10.1038/ng.3859
Source DB: PubMed Journal: Nat Genet ISSN： 1061-4036 Impact factor: 38.330