Urszula Kaźmierczak1, Dariusz Banaś2,3, Janusz Braziewicz2,3, Iwona Buraczewska4, Joanna Czub2, Marian Jaskóła5, Łukasz Kaźmierczak5, Andrzej Korman5, Marcin Kruszewski4,6, Anna Lankoff4,7, Halina Lisowska7, Marta Nesteruk1, Zygmunt Szefliński8, Maria Wojewódzka4. 1. Faculty of Physics, University of Warsaw, ul. Hoża 69, 00-681 Warsaw, Poland. 2. Institute of Physics, Jan Kochanowski University, ul. Świętokrzyska 15, 25-406 Kielce, Poland. 3. Holycross Cancer Center, ul. Świętokrzyska 15, 25-406 Kielce, Poland. 4. Institute of Nuclear Chemistry and Technology, ul. Dorodna 16, 03-195 Warsaw, Poland. 5. National Centre for Nuclear Research, ul. Andrzeja Sołtana 7, 05-400 Otwock, Świerk, Poland. 6. Institute of Rural Health, ul. Jaczewskiego 2, 20-090 Lublin, Poland. 7. Institute of Biology, Jan Kochanowski University, ul. Świętokrzyska 15, 25-406 Kielce, Poland. 8. Heavy Ion Laboratory, University of Warsaw, ul. Pasteura 5A, 02-093 Warsaw, Poland.
Abstract
AIM: Investigation of the bystander effect in Chinese Hamster Ovary cells (CHO-K1) co-cultured with cells irradiated in the dose range of 0.1-4 Gy of high LET 12C ions and X-rays. BACKGROUND: The radiobiological effects of charged heavy particles on a cellular or molecular level are of fundamental importance in the field of biomedical applications, especially in hadron therapy and space radiation biology. MATERIALS AND METHODS: A heavy ion 12C beam from the Heavy Ion Laboratory of the University of Warsaw (HIL) was used to irradiate CHO-K1 cells. Cells were seeded in Petri dishes specially designed for irradiation purposes. Immediately after irradiation, cells were transferred into transwell culture insert dishes to enable co-culture of irradiated and non-irradiated cells. Cells from the membrane and well shared the medium but could not touch each other. To study bystander effects, a clonogenic survival assay was performed. RESULTS: The survival fraction of cells co-cultured with cells irradiated with 12C ions and X-rays was not reduced. CONCLUSIONS: The bystander effect was not observed in these studies.
AIM: Investigation of the bystander effect in Chinese Hamster Ovary cells (CHO-K1) co-cultured with cells irradiated in the dose range of 0.1-4 Gy of high LET 12C ions and X-rays. BACKGROUND: The radiobiological effects of charged heavy particles on a cellular or molecular level are of fundamental importance in the field of biomedical applications, especially in hadron therapy and space radiation biology. MATERIALS AND METHODS: A heavy ion 12C beam from the Heavy Ion Laboratory of the University of Warsaw (HIL) was used to irradiate CHO-K1 cells. Cells were seeded in Petri dishes specially designed for irradiation purposes. Immediately after irradiation, cells were transferred into transwell culture insert dishes to enable co-culture of irradiated and non-irradiated cells. Cells from the membrane and well shared the medium but could not touch each other. To study bystander effects, a clonogenic survival assay was performed. RESULTS: The survival fraction of cells co-cultured with cells irradiated with 12C ions and X-rays was not reduced. CONCLUSIONS: The bystander effect was not observed in these studies.
Authors: Marianne B Sowa; Wilfried Goetz; Janet E Baulch; Dinah N Pyles; Jaroslaw Dziegielewski; Susannah Yovino; Andrew R Snyder; Sonia M de Toledo; Edouard I Azzam; William F Morgan Journal: Int J Radiat Biol Date: 2010-02 Impact factor: 2.694