Literature DB >> 2843666

Involvement of human papillomavirus type 8 genes E6 and E7 in transformation and replication.

T Iftner1, S Bierfelder, Z Csapo, H Pfister.   

Abstract

We investigated the transforming activity of human papillomavirus type 8 (HPV8) by expressing all early open reading frames from a heterologous promoter in different rodent fibroblast lines. Morphological transformation was observed only in G418-selected mouse C127 and Rat 1 cells containing an intact E6-coding region. E6 of HPV8 did not transform NIH 3T3 cells as did E6 of bovine papillomavirus type 1. C127 cells transformed by E6 were anchorage independent and had a reduced serum requirement but did not form tumors in nude mice. E7 of HPV8 showed no transforming potential, in contrast to E7 of HPV18 and HPV16. It was, however, able to complement an E7 mutant of bovine papillomavirus type 1 with a defect in high-copy-number DNA maintenance. The data indicate that the expression of the HPV8 E6 open reading frame is sufficient to induce morphological transformation in rodent fibroblasts, whereas E7 is involved in the replication of the viral DNA.

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Year:  1988        PMID： 2843666      PMCID： PMC253507          DOI： 10.1128/JVI.62.10.3655-3661.1988

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  35 in total

1.  The E6-E7 region of human papillomavirus type 18 is sufficient for transformation of NIH 3T3 and rat-1 cells.

Authors:  M A Bedell; K H Jones; L A Laimins
Journal:  J Virol       Date:  1987-11       Impact factor: 5.103

2.  Human papillomavirus type 16 open reading frame E7 encodes a transforming gene for rat 3Y1 cells.

Authors:  T Kanda; A Furuno; K Yoshiike
Journal:  J Virol       Date:  1988-02       Impact factor: 5.103

3.  Helper-independent transformation by unintegrated Harvey sarcoma virus DNA.

Authors:  D R Lowy; E Rands; E M Scolnick
Journal:  J Virol       Date:  1978-05       Impact factor: 5.103

4.  A new technique for the assay of infectivity of human adenovirus 5 DNA.

Authors:  F L Graham; A J van der Eb
Journal:  Virology       Date:  1973-04       Impact factor: 3.616

5.  Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter.

Authors:  P J Southern; P Berg
Journal:  J Mol Appl Genet       Date:  1982

6.  Sequencing end-labeled DNA with base-specific chemical cleavages.

Authors:  A M Maxam; W Gilbert
Journal:  Methods Enzymol       Date:  1980       Impact factor: 1.600

7.  Left ventricular ultrastructure in pulmonary stenosis and in tetralogy of Fallot.

Authors:  M Toussaint; C Planché; D Duboc; A Pfister; C Da Lage; F Guérin
Journal:  Virchows Arch A Pathol Anat Histopathol       Date:  1987

8.  Characterization of a human papillomavirus from epidermodysplasia verruciformis lesions of a patient from Upper-volta.

Authors:  H Pfister; F Nürnberger; L Gissmann; H zur Hausen
Journal:  Int J Cancer       Date:  1981-05-15       Impact factor: 7.396

9.  Murine sarcoma and leukemia viruses: assay using clonal lines of contact-inhibited mouse cells.

Authors:  J L Jainchill; S A Aaronson; G J Todaro
Journal:  J Virol       Date:  1969-11       Impact factor: 5.103

10.  Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.

Authors:  J M Chirgwin; A E Przybyla; R J MacDonald; W J Rutter
Journal:  Biochemistry       Date:  1979-11-27       Impact factor: 3.162
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  17 in total

1.  In vitro expressed HPV 8 E6 protein does not bind p53.

Authors:  G Steger; H Pfister
Journal:  Arch Virol       Date:  1992       Impact factor: 2.574

2.  The E6 protein of the cutaneous human papillomavirus type 8 can stimulate the viral early and late promoters by distinct mechanisms.

Authors:  Andreas Müller-Schiffmann; Julia Beckmann; Gertrud Steger
Journal:  J Virol       Date:  2006-09       Impact factor: 5.103

3.  Cell-type-specific separate regulation of the E6 and E7 promoters of human papillomavirus type 6a by the viral transcription factor E2.

Authors:  B Rapp; A Pawellek; F Kraetzer; M Schaefer; C May; K Purdie; K Grassmann; T Iftner
Journal:  J Virol       Date:  1997-09       Impact factor: 5.103

4.  Skin hyperproliferation and susceptibility to chemical carcinogenesis in transgenic mice expressing E6 and E7 of human papillomavirus type 38.

Authors:  Wen Dong; Ulrich Kloz; Rosita Accardi; Sandra Caldeira; Wei-Min Tong; Zhao-Qi Wang; Lars Jansen; Matthias Dürst; Bakary S Sylla; Lutz Gissmann; Massimo Tommasino
Journal:  J Virol       Date:  2005-12       Impact factor: 5.103

Review 5.  Human papillomavirus and the development of non-melanoma skin cancer.

Authors:  C A Harwood; J M McGregor; C M Proby; J Breuer
Journal:  J Clin Pathol       Date:  1999-04       Impact factor: 3.411

6.  Prevalence of antibodies to human papillomavirus type 8 in human sera.

Authors:  G Steger; M Olszewsky; E Stockfleth; H Pfister
Journal:  J Virol       Date:  1990-09       Impact factor: 5.103

Review 7.  Viruses associated with human cancer.

Authors:  Margaret E McLaughlin-Drubin; Karl Munger
Journal:  Biochim Biophys Acta       Date:  2007-12-23

8.  Transforming properties of the cottontail rabbit papillomavirus oncoproteins Le6 and SE6 and of the E8 protein.

Authors:  J B Harry; F O Wettstein
Journal:  J Virol       Date:  1996-06       Impact factor: 5.103

9.  Comparison of the properties of the E6 and E7 genes of low- and high-risk cutaneous papillomaviruses reveals strongly transforming and high Rb-binding activity for the E7 protein of the low-risk human papillomavirus type 1.

Authors:  A Schmitt; J B Harry; B Rapp; F O Wettstein; T Iftner
Journal:  J Virol       Date:  1994-11       Impact factor: 5.103

10.  Identification of three transforming proteins encoded by cottontail rabbit papillomavirus.

Authors:  C Meyers; J Harry; Y L Lin; F O Wettstein
Journal:  J Virol       Date:  1992-03       Impact factor: 5.103
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