Literature DB >> 284357

Emetine resistance in Chinese hamster cells is linked genetically with an altered 40S ribosomal subunit protein, S20.

D Boersma, S M McGill, J W Mollenkamp, D J Roufa.   

Abstract

Emr-2 is an emetine-resistant (Emr) Chinese hamster ovary cell mutant that contains a single electrophoretically altered ribosomal protein, a component of its 40S ribosomal subunit [Boersma, D., McGill, S., Mollenkamp, J. & Roufa, D.J. (1979) J. Biol. Chem. 254, in press]. This report describes a genetic experiment designed to test linkage between the genes that specify the altered ribosomal protein, S20*, and emetine resistance in Emr clones that segregated from cultures of Emr-2 cells hybridized with emetine-sensitive cells. The data described indicate that Emr and S20* phenotypes are due to mutations linked to the same chromosome in the Chinese hamster genome; most likely they are due to the same mutation. The data also confirm earlier speculations by others that the Emr locus in Chinese hamster cells is hemizygous.

Entities:  

Mesh：

Substances：

Year:  1979        PMID： 284357      PMCID： PMC382950          DOI： 10.1073/pnas.76.1.415

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  13 in total

1.  Derivation of TK- clones from revertant TK+ mammalian cells.

Authors:  D J Roufa; B N Sadow; C T Caskey
Journal:  Genetics       Date:  1973-11       Impact factor: 4.562

2.  Non-linkage of induced mutations in Chinese hamster cells.

Authors:  L A Chasin
Journal:  Nat New Biol       Date:  1972-11-08

3.  Organization of ribosomal protein genes in Escherichia coli. I. Physical structure of DNA from transducing lambda phages carrying genes from the aroE-str region.

Authors:  M Fiandt; W Szybalski; F R Blattner; S R Jaskunas; L Lindahl; M Nomura
Journal:  J Mol Biol       Date:  1976-09-25       Impact factor: 5.469

4.  Chromosome stability in CHO cells.

Authors:  R G Worton; C C Ho; C Duff
Journal:  Somatic Cell Genet       Date:  1977-01

5.  Improved techniques for the induction of mammalian cell hybridization by polyethylene glycol.

Authors:  R L Davidson; P S Gerald
Journal:  Somatic Cell Genet       Date:  1976-03

6.  8-Azaguanine resistance in mammalian cells. I. Hypoxanthine-guanine phosphoribosyltransferase.

Authors:  F D Gillin; D J Roufa; A L Beaudet; C T Caskey
Journal:  Genetics       Date:  1972-10       Impact factor: 4.562

7.  The isolation and preliminary characterization of somatic cell mutants resistant to the protein synthesis inhibitor-emetine.

Authors:  R S Gupta; L Siminovitch
Journal:  Cell       Date:  1976-10       Impact factor: 41.582

8.  The molecular basis of emetine resistance in Chinese hamster ovary cells: alteration in the 40S ribosomal subunit.

Authors:  R S Gupta; L Siminovitch
Journal:  Cell       Date:  1977-01       Impact factor: 41.582

9.  Evidence for functional hemizygosity at the Emtr locus in CHO cells through segregation analysis.

Authors:  R S Gupta; D Y Chan; L Siminovitch
Journal:  Cell       Date:  1978-08       Impact factor: 41.582

10.  Chromosome loss is responsible for segregation at the HPRT locus in Chinese hamster cell hybrids.

Authors:  S A Farrell; R G Worton
Journal:  Somatic Cell Genet       Date:  1977-09
View more
  15 in total

1.  Sensitivity of a mutator gene in Chinese hamster ovary cell to deoxynucleoside triphosphate pool alterations.

Authors:  M Meuth
Journal:  Mol Cell Biol       Date:  1981-07       Impact factor: 4.272

2.  A Drosophila ribosomal protein functions in mammalian cells.

Authors:  C G Maki; D D Rhoads; J J Diaz; D J Roufa
Journal:  Mol Cell Biol       Date:  1990-09       Impact factor: 4.272

3.  Next-nucleotide effects in mutations driven by DNA precursor pool imbalances at the aprt locus of Chinese hamster ovary cells.

Authors:  G Phear; J Nalbantoglu; M Meuth
Journal:  Proc Natl Acad Sci U S A       Date:  1987-07       Impact factor: 11.205

4.  Resistance against cycloheximide in cell lines from Chinese hamster and human cells is conferred by the large subunit of cytoplasmic ribosomes.

Authors:  H Pöche; S Zakrzewski; K H Nierhaus
Journal:  Mol Gen Genet       Date:  1979-09

5.  Identification and characterization of a third complementation group of emetine-resistant Chinese hamster cell mutants.

Authors:  J J Wasmuth; J M Hill; L S Vock
Journal:  Mol Cell Biol       Date:  1981-01       Impact factor: 4.272

6.  Genetic and biochemical distinction among Chinese hamster cell emtA, emtB, and emtC mutants.

Authors:  S Chang; J J Wasmuth
Journal:  Mol Cell Biol       Date:  1983-03       Impact factor: 4.272

7.  Emetine resistance in chinese hamster ovary cells is associated with an altered ribosomal protein S14 mRNA.

Authors:  J J Madjar; K Nielsen-Smith; M Frahm; D J Roufa
Journal:  Proc Natl Acad Sci U S A       Date:  1982-02       Impact factor: 11.205

8.  Primary structure of human ribosomal protein S14 and the gene that encodes it.

Authors:  D D Rhoads; A Dixit; D J Roufa
Journal:  Mol Cell Biol       Date:  1986-08       Impact factor: 4.272

9.  A cloned human ribosomal protein gene functions in rodent cells.

Authors:  D D Rhoads; D J Roufa
Journal:  Mol Cell Biol       Date:  1987-10       Impact factor: 4.272

10.  Emetine resistance of Chinese hamster cells: structures of wild-type and mutant ribosomal protein S14 mRNAs.

Authors:  D D Rhoads; D J Roufa
Journal:  Mol Cell Biol       Date:  1985-07       Impact factor: 4.272
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.