Literature DB >> 4345996

8-Azaguanine resistance in mammalian cells. I. Hypoxanthine-guanine phosphoribosyltransferase.

F D Gillin, D J Roufa, A L Beaudet, C T Caskey.   

Abstract

Chinese hamster cells were treated with ethyl methanesulfonate or N-methyl-N'-nitro-N-nitrosoguanidine, and mutants resistant to 8-azaguanine were selected and characterized. Hypoxanthine-guanine phosphoribosyltransferase activity of sixteen mutants is extremely negative, making them suitable for reversion to HGPRTase(+). Ten of the extremely negative mutants revert at a frequency higher than 10(-7) suggesting their point mutational character. The remaining mutants have demonstrable HGPRTase activity and are not useful for reversion analysis. Five of these mutants have < 2% HGPRTase and are presumably also HGPRTase point mutants. The remaining 14 mutants utilize exogenous hypoxanthine for nucleic acid synthesis poorly, and possess 20-150% of wild-type HGPRTase activity in in vitro. Their mechanism of 8-azaguanine resistance is not yet defined.

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Year:  1972        PMID： 4345996      PMCID： PMC1212824     

Source DB:  PubMed          Journal:  Genetics        ISSN： 0016-6731            Impact factor:   4.562


  14 in total

1.  PSEUDOFEEDBACK INHIBITION OF PURINE SYNTHESIS BY 6-MERCAPTOPURINE RIBONUCLEOTIDE AND OTHER PURINE ANALOGUES.

Authors:  R J MCCOLLISTER; W R GILBERT; D M ASHTON; J B WYNGAARDEN
Journal:  J Biol Chem       Date:  1964-05       Impact factor: 5.157

2.  THE ENZYMOLOGY OF FEEDBACK INHIBITION OF GLUTAMINE PHOSPHORIBOSYLPYROPHOSPHATE AMIDOTRANSFERASE BY PURINE RIBONUCLEOTIDES.

Authors:  C T CASKEY; D M ASHTON; J B WYNGAARDEN
Journal:  J Biol Chem       Date:  1964-08       Impact factor: 5.157

3.  BIOCHEMICALLY MARKED VARIANTS OF THE SYRIAN HAMSTER FIBROBLAST CELL LINE BHK21 AND ITS DERIVATIVES.

Authors:  H SUBAK-SHARPE
Journal:  Exp Cell Res       Date:  1965-04       Impact factor: 3.905

4.  The regulation of activity of phosphoribosyl-pyrophosphate amidotransferase by purine ribonacleotides: a potential feedback control of purine biosyntoesis.

Authors:  J B WYNGAARDEN; D M ASHTON
Journal:  J Biol Chem       Date:  1959-06       Impact factor: 5.157

5.  Regulation of the biosynthesis of purine nucleotides in Schizosaccharomyces pombe. I. Properties of the phosphoribosylpyrophosphate: glutamine amidotransferase of the wild strain and of a mutant desensitized towards feedback modifiers.

Authors:  M Nagy
Journal:  Biochim Biophys Acta       Date:  1970-03-18

6.  Defective guanine uptake in an 8-azaguanine-resistant mutant of Salmonella typhimurium.

Authors:  J H Thakar; G P Kalle
Journal:  J Bacteriol       Date:  1968-02       Impact factor: 3.490

7.  Classification of aminotransferase (C gene) mutants in the histidine operon.

Authors:  H J Whitfield; R G Martin; B N Ames
Journal:  J Mol Biol       Date:  1966-11-14       Impact factor: 5.469

8.  The relationship of gene structure and protein structure of iso-I-cytochrome c from yeast.

Authors:  F Sherman; J W Stewart; J H Parker; G J Putterman; B B Agrawal; E Margoliash
Journal:  Symp Soc Exp Biol       Date:  1970

9.  Inheritance of purine phosphoribosyltransferases in man.

Authors:  J F Henderson; W N Kelley; F M Rosenbloom; J E Seegmiller
Journal:  Am J Hum Genet       Date:  1969-01       Impact factor: 11.025

10.  Decreased feedback inhibition in a 6-(methylmercapto)purine ribonucleoside-resistant tumor.

Authors:  J F Henderson; I C Caldwell; A R Paterson
Journal:  Cancer Res       Date:  1967-10       Impact factor: 12.701
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  66 in total

1.  Dissociation of cytotoxicity and DNA cleavage activity induced by topoisomerase II-reactive intercalating agents in hamster-human somatic cell hybrids.

Authors:  B S Glisson; A M Killary; P Merta; W E Ross; J Siciliano; M J Siciliano
Journal:  Cancer Chemother Pharmacol       Date:  1992       Impact factor: 3.333

2.  5-bromodeoxyuridine-DNA strand symmetry and the repair of photolytic breaks in Chinese hamster cell chromosomes.

Authors:  D J Roufa
Journal:  Proc Natl Acad Sci U S A       Date:  1976-11       Impact factor: 11.205

3.  Three distinct envelope domains, variably present in subgroup B feline leukemia virus recombinants, mediate Pit1 and Pit2 receptor recognition.

Authors:  S Boomer; M Eiden; C C Burns; J Overbaugh
Journal:  J Virol       Date:  1997-11       Impact factor: 5.103

4.  Escape variants of the XPR1 gammaretrovirus receptor are rare due to reliance on a splice donor site and a short hypervariable loop.

Authors:  Xiaoyu Lu; Carrie Martin; Christelle Bouchard; Christine A Kozak
Journal:  Virology       Date:  2014-08-20       Impact factor: 3.616

5.  Genes controlling receptors for ecotropic and xenotropic type C virus in Mus cervicolor and Mus musculus.

Authors:  T H Marshall; U R Rapp
Journal:  J Virol       Date:  1979-02       Impact factor: 5.103

6.  Emetine resistance in chinese hamster ovary cells is associated with an altered ribosomal protein S14 mRNA.

Authors:  J J Madjar; K Nielsen-Smith; M Frahm; D J Roufa
Journal:  Proc Natl Acad Sci U S A       Date:  1982-02       Impact factor: 11.205

7.  Assignment of adenosine deaminase complexing protein (ADCP) gene(s) to human chromosome 2 in rodent-human somatic cell hybrids.

Authors:  E Herbschleb-Voogt; K H Grzeschik; P L Pearson; P Meera Khan
Journal:  Hum Genet       Date:  1981       Impact factor: 4.132

8.  Adenovirus-induced mutations at the hypoxanthine phosphoribosyltransferase locus of Chinese hamster cells.

Authors:  C Marengo; M Mbikay; J Weber; J P Thirion
Journal:  J Virol       Date:  1981-04       Impact factor: 5.103

9.  Increased removal of 3-alkyladenine reduces the frequencies of hprt mutations induced by methyl- and ethylmethanesulfonate in Chinese hamster fibroblast cells.

Authors:  A Klungland; M Bjørås; E Hoff; E Seeberg
Journal:  Nucleic Acids Res       Date:  1994-05-11       Impact factor: 16.971

10.  Reduction of the toxicity and mutagenicity of alkylating agents in mammalian cells harboring the Escherichia coli alkyltransferase gene.

Authors:  J Brennand; G P Margison
Journal:  Proc Natl Acad Sci U S A       Date:  1986-09       Impact factor: 11.205
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