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Literature DB >> 1528894

Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector.

H Shizuya¹, B Birren, U J Kim, V Mancino, T Slepak, Y Tachiiri, M Simon.

Abstract

A bacterial cloning system for mapping and analysis of complex genomes has been developed. The BAC system (for bacterial artificial chromosome) is based on Escherichia coli and its single-copy plasmid F factor. It is capable of maintaining human genomic DNA fragments of greater than 300 kilobase pairs. Individual clones of human DNA appear to be maintained with a high degree of structural stability in the host, even after 100 generations of serial growth. Because of high cloning efficiency, easy manipulation of the cloned DNA, and stable maintenance of inserted DNA, the BAC system may facilitate construction of DNA libraries of complex genomes with fuller representation and subsequent rapid analysis of complex genomic structure.

Entities: Species

Mesh：

Year: 1992 PMID： 1528894 PMCID： PMC50007 DOI： 10.1073/pnas.89.18.8794

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

14 in total

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Journal: Genomics Date: 1991-11 Impact factor: 5.736

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Journal: Science Date: 1987-05-15 Impact factor: 47.728

6. Analysis of cosmids using linearization by phage lambda terminase.

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Journal: Proc Natl Acad Sci U S A Date: 1990-01 Impact factor: 11.205

8. A recB recC sbcB recJ host prevents recA-independent deletions in recombinant cosmid DNA propagated in Escherichia coli.

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Journal: J Bacteriol Date: 1989-02 Impact factor: 3.490

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Authors: U J Kim; H Shizuya; P J de Jong; B Birren; M I Simon
Journal: Nucleic Acids Res Date: 1992-03-11 Impact factor: 16.971

10. Human Xq24-Xq28: approaches to mapping with yeast artificial chromosomes.

Authors: M Wada; R D Little; F Abidi; G Porta; T Labella; T Cooper; G Della Valle; M D'Urso; D Schlessinger
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408 in total

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3. Construction and characterization of a common bean bacterial artificial chromosome library.

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5. Efficiency and stability of high molecular weight DNA transformation: an analysis in tomato.

Authors: A Frary; C M Hamilton
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6. Locus-specific contig assembly in highly-duplicated genomes, using the BAC-RF method.

Authors: Y R Lin; X Draye; X Qian; S Ren; L H Zhu; J Tomkins; R A Wing; Z Li; A H Paterson
Journal: Nucleic Acids Res Date: 2000-04-01 Impact factor: 16.971

7. Cloning the soil metagenome: a strategy for accessing the genetic and functional diversity of uncultured microorganisms.

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