Literature DB >> 2833195

Cloning and expression in Escherichia coli of the Clostridium thermoaceticum gene encoding thermostable formyltetrahydrofolate synthetase.

C R Lovell1, A Przybyla, L G Ljungdahl.   

Abstract

Formyltetrahydrofolate synthetase (FTHFS) (EC 6.3.4.3), a thermostable protein of four identical subunits from Clostridium thermoaceticum was cloned into Escherichia coli SK1592. The clone (CRL47) contained a 9.5 kb EcoRI fragment of C. thermoaceticum DNA ligated into pBR322. It produced catalytically active, thermostable FTHFS, that was not found in E. coli SK1592 containing native pBR322. The identity of the expressed enzyme was confirmed by specific binding of rabbit polyclonal anti-FTHFS serum produced against C. thermoaceticum FTHFS. The specific activities (mumol.min-1.mg-1) of FTHFS in cell free extracts of CRL47 were 28-89 when assayed at 50 degrees C and pH 8. This was from 3-10-fold higher than in C. thermoaceticum extracts. FTHFS was purified to homogeneity from CRL47. The purified enzyme behaved during electrophoresis and gel chromatography and it had similar specific activity and thermostability as the enzyme purified from C. thermoaceticum.

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Year:  1988        PMID： 2833195     DOI： 10.1007/BF00411642

Source DB:  PubMed          Journal:  Arch Microbiol        ISSN： 0302-8933            Impact factor:   2.552


  25 in total

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5.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

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6.  Purification, stability, and composition of formyltetrahydrofolate synthetase from Clostridium thermoaceticum.

Authors:  L Ljungdahl; J M Brewer; S H Neece; T Fairwell
Journal:  J Biol Chem       Date:  1970-09-25       Impact factor: 5.157

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8.  Chemical modification of cysteine and tyrosine residues in formyltetrahydrofolate synthetase from Clostridium thermoaceticum.

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9.  Cloning and expression in Escherichia coli of the gene for 10-formyltetrahydrofolate synthetase from Clostridium acidiurici ("Clostridium acidi-urici").

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Authors:  G Salvesen; D Farley; J Shuman; A Przybyla; C Reilly; J Travis
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  9 in total

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7.  The complete genome sequence of Moorella thermoacetica (f. Clostridium thermoaceticum).

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9.  A genome-guided analysis of energy conservation in the thermophilic, cytochrome-free acetogenic bacterium Thermoanaerobacter kivui.

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  9 in total

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