Literature DB >> 3856838

Base composition-independent hybridization in tetramethylammonium chloride: a method for oligonucleotide screening of highly complex gene libraries.

W I Wood, J Gitschier, L A Lasky, R M Lawn.   

Abstract

An oligonucleotide hybridization procedure has been developed that eliminates the preferential melting of A X T versus G X C base pairs, allowing the stringency of the hybridization to be controlled as a function of probe length only. This technique, which uses tetramethylammonium chloride, is especially helpful whenever a highly complex library is screened with a pool of oligonucleotide probes, which usually vary widely in base composition. The procedure can also be applied advantageously whenever an exact match to an oligonucleotide probe is desired, such as in screening for clones having as little as a single-base alteration generated by in vitro mutagenesis.

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Year:  1985        PMID： 3856838      PMCID： PMC397316          DOI： 10.1073/pnas.82.6.1585

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  15 in total

1.  Hybridization with synthetic oligonucleotides.

Authors:  J W Szostak; J I Stiles; B K Tye; P Chiu; F Sherman; R Wu
Journal:  Methods Enzymol       Date:  1979       Impact factor: 1.600

2.  DNA melting temperatures and renaturation rates in concentrated alkylammonium salt solutions.

Authors:  J M Orosz; J G Wetmur
Journal:  Biopolymers       Date:  1977-06       Impact factor: 2.505

3.  Effects of microscopic and macroscopic viscosity on the rate of renaturation of DNA.

Authors:  C T Chang; T C Hain; J R Hutton; J G Wetmur
Journal:  Biopolymers       Date:  1974       Impact factor: 2.505

4.  The binding of small cations to deoxyribonucleic acid. Nucleotide specificity.

Authors:  J T Shapiro; B S Stannard; G Felsenfeld
Journal:  Biochemistry       Date:  1969-08       Impact factor: 3.162

5.  The single-copy DNA sequence polymorphism of the sea urchin Strongylocentrotus purpuratus.

Authors:  R J Britten; A Cetta; E H Davidson
Journal:  Cell       Date:  1978-12       Impact factor: 41.582

6.  Alteration of the relative stability of dA-dT and dG-dC base pairs in DNA.

Authors:  W B Melchior; P H Von Hippel
Journal:  Proc Natl Acad Sci U S A       Date:  1973-02       Impact factor: 11.205

7.  Hybridization of synthetic oligodeoxyribonucleotides to phi chi 174 DNA: the effect of single base pair mismatch.

Authors:  R B Wallace; J Shaffer; R F Murphy; J Bonner; T Hirose; K Itakura
Journal:  Nucleic Acids Res       Date:  1979-08-10       Impact factor: 16.971

8.  Synthesis of oligonucleotides on cellulose by a phosphotriester method.

Authors:  R Crea; T Horn
Journal:  Nucleic Acids Res       Date:  1980-05-24       Impact factor: 16.971

9.  Human leukocyte interferon produced by E. coli is biologically active.

Authors:  D V Goeddel; E Yelverton; A Ullrich; H L Heyneker; G Miozzari; W Holmes; P H Seeburg; T Dull; L May; N Stebbing; R Crea; S Maeda; R McCandliss; A Sloma; J M Tabor; M Gross; P C Familletti; S Pestka
Journal:  Nature       Date:  1980-10-02       Impact factor: 49.962

10.  The use of synthetic oligonucleotides as hybridization probes. II. Hybridization of oligonucleotides of mixed sequence to rabbit beta-globin DNA.

Authors:  R B Wallace; M J Johnson; T Hirose; T Miyake; E H Kawashima; K Itakura
Journal:  Nucleic Acids Res       Date:  1981-02-25       Impact factor: 16.971
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  288 in total

1.  Melanocortin-1 receptor polymorphisms and risk of melanoma: is the association explained solely by pigmentation phenotype?

Authors:  J S Palmer; D L Duffy; N F Box; J F Aitken; L E O'Gorman; A C Green; N K Hayward; N G Martin; R A Sturm
Journal:  Am J Hum Genet       Date:  2000-01       Impact factor: 11.025

2.  Cloning of fibA, encoding an immunogenic subunit of the fibril-like surface structure of Peptostreptococcus micros.

Authors:  B H Kremer; J J Bijlsma; J G Kusters; J de Graaff; T J van Steenbergen
Journal:  J Bacteriol       Date:  1999-04       Impact factor: 3.490

3.  A bead-based method for multiplexed identification and quantitation of DNA sequences using flow cytometry.

Authors:  A Spiro; M Lowe; D Brown
Journal:  Appl Environ Microbiol       Date:  2000-10       Impact factor: 4.792

4.  Modification of DNA duplexes to smooth their thermal stability independently of their base content for DNA sequencing by hybridization.

Authors:  H K Nguyen; P Auffray; U Asseline; D Dupret; N T Thuong
Journal:  Nucleic Acids Res       Date:  1997-08-01       Impact factor: 16.971

5.  A novel murine P-450 gene, Cyp4a14, is part of a cluster of Cyp4a and Cyp4b, but not of CYP4F, genes in mouse and humans.

Authors:  Y M Heng; C S Kuo; P S Jones; R Savory; R M Schulz; S R Tomlinson; T J Gray; D R Bell
Journal:  Biochem J       Date:  1997-08-01       Impact factor: 3.857

6.  Thermodynamic treatment of oligonucleotide duplex-simplex equilibria.

Authors:  Richard Owczarzy; Isard Dunietz; Mark A Behlke; Irving M Klotz; Joseph A Walder
Journal:  Proc Natl Acad Sci U S A       Date:  2003-12-01       Impact factor: 11.205

7.  Global haplotype diversity in the human insulin gene region.

Authors:  John D H Stead; Matthew E Hurles; Alec J Jeffreys
Journal:  Genome Res       Date:  2003-09       Impact factor: 9.043

8.  Isolation of a cDNA clone of the 14-kDa subunit of the signal recognition particle by cross-hybridization of differently primed polymerase chain reactions.

Authors:  K Strub; P Walter
Journal:  Proc Natl Acad Sci U S A       Date:  1989-12       Impact factor: 11.205

9.  Nonsense mutation causing steroid 21-hydroxylase deficiency.

Authors:  H Globerman; M Amor; K L Parker; M I New; P C White
Journal:  J Clin Invest       Date:  1988-07       Impact factor: 14.808

10.  The putative testis-determining factor and related genes are expressed as discrete-sized transcripts in adult gonadal and somatic tissues.

Authors:  Y F Lau; K M Chan
Journal:  Am J Hum Genet       Date:  1989-12       Impact factor: 11.025
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