Literature DB >> 2830596

Homologous recombination of SV40 DNA in COS7 cells occurs with high frequency in a gene dose independent fashion.

W Rommerskirch1, I Graeber, M Grässmann, A Grässmann.   

Abstract

Homologous recombination between microinjected SV40 DNA fragments and endogenous SV40 DNA in COS7 cells was analysed by immunofluorescence staining and DNA blotting. Time course experiments revealed that recombination between the transferred (trans) DNA and the chromosomal DNA occurred about 8 hours after microinjection with high efficiency in a gene dose independent fashion. Deletions of up to 1018 basepairs (bp) within the early or the late SV40 region were efficiently repaired after the transfer of linear but not of circular DNA molecules. A 22 bp homology between the trans DNA and the endogenous DNA was sufficient to initiate recombination but 14 nonhomologous bp at one open end of the SV40 DNA fragments hindered gap repair.

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Year:  1988        PMID： 2830596      PMCID： PMC334729          DOI： 10.1093/nar/16.3.941

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  31 in total

1.  Nucleotide sequence analysis of viable deletion mutants lacking segments of the simian virus 40 genome coding for small t antigen.

Authors:  B Thimmappaya; T Shenk
Journal:  J Virol       Date:  1979-06       Impact factor: 5.103

2.  A new technique for the assay of infectivity of human adenovirus 5 DNA.

Authors:  F L Graham; A J van der Eb
Journal:  Virology       Date:  1973-04       Impact factor: 3.616

3.  SV40-transformed simian cells support the replication of early SV40 mutants.

Authors:  Y Gluzman
Journal:  Cell       Date:  1981-01       Impact factor: 41.582

4.  Sequencing end-labeled DNA with base-specific chemical cleavages.

Authors:  A M Maxam; W Gilbert
Journal:  Methods Enzymol       Date:  1980       Impact factor: 1.600

5.  "Early" simian-virus-40-specific RNA contains information for tumor antigen formation and chromatin replication.

Authors:  M Graessmann; A Graessman
Journal:  Proc Natl Acad Sci U S A       Date:  1976-02       Impact factor: 11.205

6.  Cellular mutation mediates T-antigen-positive revertant cells resistant to simian virus 40 transformation but not to retransformation by polyomavirus and adenovirus type 2.

Authors:  M Bauer; E Guhl; M Graessmann; A Graessmann
Journal:  J Virol       Date:  1987-06       Impact factor: 5.103

7.  Chromatin structure is required to block transcription of the methylated herpes simplex virus thymidine kinase gene.

Authors:  G Buschhausen; B Wittig; M Graessmann; A Graessmann
Journal:  Proc Natl Acad Sci U S A       Date:  1987-03       Impact factor: 11.205

8.  Multiple point mutations affecting the simian virus 40 enhancer.

Authors:  H Weiher; M König; P Gruss
Journal:  Science       Date:  1983-02-11       Impact factor: 47.728

9.  Simian virus 40 small-t protein is required for loss of actin cable networks in rat cells.

Authors:  A Graessmann; M Graessmann; R Tjian; W C Topp
Journal:  J Virol       Date:  1980-03       Impact factor: 5.103

10.  Homologous and nonhomologous recombination in monkey cells.

Authors:  S Subramani; P Berg
Journal:  Mol Cell Biol       Date:  1983-06       Impact factor: 4.272
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  13 in total

Review 1.  Manipulating the mammalian genome by homologous recombination.

Authors:  K M Vasquez; K Marburger; Z Intody; J H Wilson
Journal:  Proc Natl Acad Sci U S A       Date:  2001-07-17       Impact factor: 11.205

2.  A chromosomal position effect on gene targeting in human cells.

Authors:  Rafael J Yáñez; Andrew C G Porter
Journal:  Nucleic Acids Res       Date:  2002-11-15       Impact factor: 16.971

3.  The frequency of gene targeting in Trypanosoma brucei is independent of target site copy number.

Authors:  Bill Wickstead; Klaus Ersfeld; Keith Gull
Journal:  Nucleic Acids Res       Date:  2003-07-15       Impact factor: 16.971

4.  Initiation of replication of the human hepatitis delta virus genome from cloned DNA: role of delta antigen.

Authors:  M Y Kuo; M Chao; J Taylor
Journal:  J Virol       Date:  1989-05       Impact factor: 5.103

5.  Gene targeting in Chinese hamster ovary cells is conservative.

Authors:  S L Pennington; J H Wilson
Journal:  Proc Natl Acad Sci U S A       Date:  1991-11-01       Impact factor: 11.205

Review 6.  Prospects for homologous recombination in human gene therapy.

Authors:  M A Vega
Journal:  Hum Genet       Date:  1991-07       Impact factor: 4.132

7.  Illegitimate and homologous recombination in mammalian cells: differential sensitivity to an inhibitor of poly(ADP-ribosylation).

Authors:  B C Waldman; A S Waldman
Journal:  Nucleic Acids Res       Date:  1990-10-25       Impact factor: 16.971

8.  Gene therapy for cancers.

Authors:  L Lashford
Journal:  Arch Dis Child       Date:  1994-12       Impact factor: 3.791

9.  The frequency of gene targeting in yeast depends on the number of target copies.

Authors:  J H Wilson; W Y Leung; G Bosco; D Dieu; J E Haber
Journal:  Proc Natl Acad Sci U S A       Date:  1994-01-04       Impact factor: 11.205

10.  The search for homology does not limit the rate of extrachromosomal homologous recombination in mammalian cells.

Authors:  A S Waldman
Journal:  Genetics       Date:  1994-02       Impact factor: 4.562
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