| Literature DB >> 28280153 |
Yue Shen1,2,3,4, Yun Wang1,4, Tai Chen1,4, Feng Gao1, Jianhui Gong1, Dariusz Abramczyk2, Roy Walker2, Hongcui Zhao1, Shihong Chen1, Wei Liu2, Yisha Luo2, Carolin A Müller5, Adrien Paul-Dubois-Taine2, Bonnie Alver2, Giovanni Stracquadanio6,7, Leslie A Mitchell8, Zhouqing Luo9, Yanqun Fan1, Baojin Zhou1, Bo Wen1, Fengji Tan1, Yujia Wang1, Jin Zi1, Zexiong Xie10, Bingzhi Li10, Kun Yang6, Sarah M Richardson6,7, Hui Jiang1, Christopher E French2, Conrad A Nieduszynski5, Romain Koszul11, Adele L Marston2, Yingjin Yuan10, Jian Wang1, Joel S Bader7,8, Junbiao Dai9, Jef D Boeke8, Xun Xu1,4, Yizhi Cai2, Huanming Yang1,3.
Abstract
Here, we report the successful design, construction, and characterization of a 770-kilobase synthetic yeast chromosome II (synII). Our study incorporates characterization at multiple levels-including phenomics, transcriptomics, proteomics, chromosome segregation, and replication analysis-to provide a thorough and comprehensive analysis of a synthetic chromosome. Our Trans-Omics analyses reveal a modest but potentially relevant pervasive up-regulation of translational machinery observed in synII, mainly caused by the deletion of 13 transfer RNAs. By both complementation assays and SCRaMbLE (synthetic chromosome rearrangement and modification by loxP-mediated evolution), we targeted and debugged the origin of a growth defect at 37°C in glycerol medium, which is related to misregulation of the high-osmolarity glycerol response. Despite the subtle differences, the synII strain shows highly consistent biological processes comparable to the native strain.Entities:
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Year: 2017 PMID： 28280153 PMCID： PMC5390853 DOI： 10.1126/science.aaf4791
Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728