| Literature DB >> 28280152 |
Yi Wu1,2, Bing-Zhi Li1,2, Meng Zhao1,2, Leslie A Mitchell3, Ze-Xiong Xie1,2, Qiu-Hui Lin1,2, Xia Wang1,2, Wen-Hai Xiao1,2, Ying Wang1,2, Xiao Zhou1,2, Hong Liu1,2, Xia Li1,2, Ming-Zhu Ding1,2, Duo Liu1,2, Lu Zhang1,2, Bao-Li Liu1,2, Xiao-Le Wu1,2, Fei-Fei Li1,2, Xiu-Tao Dong1,2, Bin Jia1,2, Wen-Zheng Zhang1,2, Guo-Zhen Jiang1,2, Yue Liu1,2, Xue Bai1,2, Tian-Qing Song1,2, Yan Chen1,2, Si-Jie Zhou1,2, Rui-Ying Zhu1,2, Feng Gao1,2, Zheng Kuang3, Xuya Wang3, Michael Shen3, Kun Yang4, Giovanni Stracquadanio4,5, Sarah M Richardson4, Yicong Lin6, Lihui Wang6, Roy Walker7, Yisha Luo7, Ping-Sheng Ma1,2, Huanming Yang8,9, Yizhi Cai7, Junbiao Dai6, Joel S Bader4, Jef D Boeke3, Ying-Jin Yuan10,2.
Abstract
Debugging a genome sequence is imperative for successfully building a synthetic genome. As part of the effort to build a designer eukaryotic genome, yeast synthetic chromosome X (synX), designed as 707,459 base pairs, was synthesized chemically. SynX exhibited good fitness under a wide variety of conditions. A highly efficient mapping strategy called pooled PCRTag mapping (PoPM), which can be generalized to any watermarked synthetic chromosome, was developed to identify genetic alterations that affect cell fitness ("bugs"). A series of bugs were corrected that included a large region bearing complex amplifications, a growth defect mapping to a recoded sequence in FIP1, and a loxPsym site affecting promoter function of ATP2 PoPM is a powerful tool for synthetic yeast genome debugging and an efficient strategy for phenotype-genotype mapping.Entities:
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Year: 2017 PMID： 28280152 PMCID： PMC5679077 DOI： 10.1126/science.aaf4706
Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728