| Literature DB >> 28280151 |
Ze-Xiong Xie1,2, Bing-Zhi Li1,2, Leslie A Mitchell3, Yi Wu1,2, Xin Qi1,2, Zhu Jin1,2, Bin Jia1,2, Xia Wang1,2, Bo-Xuan Zeng1,2, Hui-Min Liu1,2, Xiao-Le Wu1,2, Qi Feng1,2, Wen-Zheng Zhang1,2, Wei Liu1,2, Ming-Zhu Ding1,2, Xia Li1,2, Guang-Rong Zhao1,2, Jian-Jun Qiao1,2, Jing-Sheng Cheng1,2, Meng Zhao1,2, Zheng Kuang3, Xuya Wang3, J Andrew Martin3, Giovanni Stracquadanio4,5, Kun Yang4, Xue Bai1,2, Juan Zhao1,2, Meng-Long Hu1,2, Qiu-Hui Lin1,2, Wen-Qian Zhang1,2, Ming-Hua Shen1,2, Si Chen1,2, Wan Su1,2, En-Xu Wang1,2, Rui Guo1,2, Fang Zhai1,2, Xue-Jiao Guo1,2, Hao-Xing Du1,2, Jia-Qing Zhu1,2, Tian-Qing Song1,2, Jun-Jun Dai1,2, Fei-Fei Li1,2, Guo-Zhen Jiang1,2, Shi-Lei Han1,2, Shi-Yang Liu1,2, Zhi-Chao Yu1,2, Xiao-Na Yang1,2, Ken Chen1,2, Cheng Hu1,2, Da-Shuai Li1,2, Nan Jia1,2, Yue Liu1,2, Lin-Ting Wang1,2, Su Wang1,2, Xiao-Tong Wei1,2, Mei-Qing Fu1,2, Lan-Meng Qu1,2, Si-Yu Xin1,2, Ting Liu1,2, Kai-Ren Tian1,2, Xue-Nan Li1,2, Jin-Hua Zhang1,2, Li-Xiang Song1,2, Jin-Gui Liu1,2, Jia-Fei Lv1,2, Hang Xu1,2, Ran Tao1,2, Yan Wang1,2, Ting-Ting Zhang1,2, Ye-Xuan Deng1,2, Yi-Ran Wang1,2, Ting Li1,2, Guang-Xin Ye1,2, Xiao-Ran Xu1,2, Zheng-Bao Xia1,2, Wei Zhang1,2, Shi-Lan Yang1,2, Yi-Lin Liu1,2, Wen-Qi Ding1,2, Zhen-Ning Liu1,2, Jun-Qi Zhu1,2, Ning-Zhi Liu1,2, Roy Walker6, Yisha Luo6, Yun Wang7, Yue Shen7, Huanming Yang7,8, Yizhi Cai6, Ping-Sheng Ma1, Chun-Ting Zhang1, Joel S Bader4, Jef D Boeke3, Ying-Jin Yuan9,2.
Abstract
Perfect matching of an assembled physical sequence to a specified designed sequence is crucial to verify design principles in genome synthesis. We designed and de novo synthesized 536,024-base pair chromosome synV in the "Build-A-Genome China" course. We corrected an initial isolate of synV to perfectly match the designed sequence using integrative cotransformation and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9)-mediated editing in 22 steps; synV strains exhibit high fitness under a variety of culture conditions, compared with that of wild-type V strains. A ring synV derivative was constructed, which is fully functional in Saccharomyces cerevisiae under all conditions tested and exhibits lower spore viability during meiosis. Ring synV chromosome can extends Sc2.0 design principles and provides a model with which to study genomic rearrangement, ring chromosome evolution, and human ring chromosome disorders.Entities:
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Year: 2017 PMID： 28280151 DOI： 10.1126/science.aaf4704
Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728