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Literature DB >> 28244989

A fully automated flow-based approach for accelerated peptide synthesis.

Alexander J Mijalis¹, Dale A Thomas², Mark D Simon¹, Andrea Adamo², Ryan Beaumont³, Klavs F Jensen², Bradley L Pentelute¹.

Abstract

Here we report a fully automated, flow-based approach to solid-phase polypeptide synthesis, with amide bond formation in 7 seconds and total synthesis times of 40 seconds per amino acid residue. Crude peptide purities and isolated yields were comparable to those for standard-batch solid-phase peptide synthesis. At full capacity, this approach can yield tens of thousands of individual 30-mer peptides per year.

Entities: Chemical

Mesh：

Substances：
Peptides

Year: 2017 PMID： 28244989 DOI： 10.1038/nchembio.2318

Source DB: PubMed Journal: Nat Chem Biol ISSN： 1552-4450 Impact factor: 15.040

16 in total

1. Applying flow chemistry: methods, materials, and multistep synthesis.

Authors: D Tyler McQuade; Peter H Seeberger
Journal: J Org Chem Date: 2013-06-20 Impact factor: 4.354

2. Flash chemistry: flow chemistry that cannot be done in batch.

Authors: Jun-ichi Yoshida; Yusuke Takahashi; Aiichiro Nagaki
Journal: Chem Commun (Camb) Date: 2013-11-04 Impact factor: 6.222

3. High-efficiency solid phase peptide synthesis (HE-SPPS).

Authors: Jonathan M Collins; Keith A Porter; Sandeep K Singh; Grace S Vanier
Journal: Org Lett Date: 2014-01-23 Impact factor: 6.005

4. On-demand continuous-flow production of pharmaceuticals in a compact, reconfigurable system.

Authors: Andrea Adamo; Rachel L Beingessner; Mohsen Behnam; Jie Chen; Timothy F Jamison; Klavs F Jensen; Jean-Christophe M Monbaliu; Allan S Myerson; Eve M Revalor; David R Snead; Torsten Stelzer; Nopphon Weeranoppanant; Shin Yee Wong; Ping Zhang
Journal: Science Date: 2016-04-01 Impact factor: 47.728

5. Color test for detection of free terminal amino groups in the solid-phase synthesis of peptides.

Authors: E Kaiser; R L Colescott; C D Bossinger; P I Cook
Journal: Anal Biochem Date: 1970-04 Impact factor: 3.365

6. Rapid total synthesis of DARPin pE59 and barnase.

Authors: Surin K Mong; Alexander A Vinogradov; Mark D Simon; Bradley L Pentelute
Journal: Chembiochem Date: 2014-03-11 Impact factor: 3.164

7. Quantitative monitoring of solid-phase peptide synthesis by the ninhydrin reaction.

Authors: V K Sarin; S B Kent; J P Tam; R B Merrifield
Journal: Anal Biochem Date: 1981-10 Impact factor: 3.365

8. Enhanced Solvation of Peptides Attached to "Solid-Phase" Resins: Straightforward Syntheses of the Elastin Sequence Pro-Gly-Val-Gly-Val-Pro-Gly-Val-Gly-Val.

Authors: Bobo Dang; Balamurugan Dhayalan; Stephen B H Kent
Journal: Org Lett Date: 2015-06-25 Impact factor: 6.005

9. Evaluation of COMU as a coupling reagent for in situ neutralization Boc solid phase peptide synthesis.

Authors: Claudia U Hjørringgaard; Andreas Brust; Paul F Alewood
Journal: J Pept Sci Date: 2012-01-17 Impact factor: 1.905

10. Solid-phase peptide synthesis: from standard procedures to the synthesis of difficult sequences.

Authors: Irene Coin; Michael Beyermann; Michael Bienert
Journal: Nat Protoc Date: 2007 Impact factor: 13.491

56 in total

Review 1. Trends in peptide drug discovery.

Authors: Markus Muttenthaler; Glenn F King; David J Adams; Paul F Alewood
Journal: Nat Rev Drug Discov Date: 2021-02-03 Impact factor: 84.694

A fully automated flow-based approach for accelerated peptide synthesis.

1. Applying flow chemistry: methods, materials, and multistep synthesis.

2. Flash chemistry: flow chemistry that cannot be done in batch.

3. High-efficiency solid phase peptide synthesis (HE-SPPS).

4. On-demand continuous-flow production of pharmaceuticals in a compact, reconfigurable system.

5. Color test for detection of free terminal amino groups in the solid-phase synthesis of peptides.

6. Rapid total synthesis of DARPin pE59 and barnase.

7. Quantitative monitoring of solid-phase peptide synthesis by the ninhydrin reaction.

8. Enhanced Solvation of Peptides Attached to "Solid-Phase" Resins: Straightforward Syntheses of the Elastin Sequence Pro-Gly-Val-Gly-Val-Pro-Gly-Val-Gly-Val.

9. Evaluation of COMU as a coupling reagent for in situ neutralization Boc solid phase peptide synthesis.

10. Solid-phase peptide synthesis: from standard procedures to the synthesis of difficult sequences.

Review 1. Trends in peptide drug discovery.

Review 2. Novel protein science enabled by total chemical synthesis.

Review 3. Reductionist Approach in Peptide-Based Nanotechnology.

4. In-solution enrichment identifies peptide inhibitors of protein-protein interactions.

Review 5. Global substrate specificity profiling of post-translational modifying enzymes.

6. A Global Minimization Toolkit for Batch-Fitting and χ² Cluster Analysis of CW-EPR Spectra.

Review 7. The Molecular Industrial Revolution: Automated Synthesis of Small Molecules.

Review 8. Towards personalized, tumour-specific, therapeutic vaccines for cancer.

9. Conformational Dynamics in Extended RGD-Containing Peptides.

10. ¹⁷O MAS NMR Correlation Spectroscopy at High Magnetic Fields.