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Literature DB >> 2822970

Expression of hepatitis A virus cDNA in Escherichia coli: antigenic VP1 recombinant protein.

R Ostermayr¹, K von der Helm, V Gauss-Müller, E L Winnacker, F Deinhardt.

Abstract

The genome of hepatitis A virus (HAV) was reverse transcribed into cDNA and molecularly cloned. cDNA clones coding for the capsid protein VP1 that carries the major HAV antigen were cloned into the expression vector pUR290 and expressed in Escherichia coli. The recombinant fusion protein reacted in an immunoblot with rabbit anti-HAV serum, suggesting that it possesses HAV antigenicity.

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Year: 1987 PMID： 2822970 PMCID： PMC255970 DOI： 10.1128/JVI.61.11.3645-3647.1987

Source DB: PubMed Journal: J Virol ISSN： 0022-538X Impact factor: 5.103

17 in total

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2. Propagation of human hepatitis A virus in cell culture in vitro.

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Journal: Proc Soc Exp Biol Med Date: 1979-02

3. Propagation of hepatitis A virus in human embryo fibroblasts.

Authors: V Gauss-Müller; G G Frösner; F Deinhardt
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Authors: A G Coulepis; G A Tannock; S A Locarnini; I D Gust
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5. Classification of hepatitis A virus as enterovirus type 72 and of hepatitis B virus as hepadnavirus type 1.

Authors: J L Melnick
Journal: Intervirology Date: 1982 Impact factor: 1.763

6. Propagation of human hepatitis A virus in a hepatoma cell line.

Authors: G G Frösner; F Deinhardt; R Scheid; V Gauss-Müller; N Holmes; V Messelberger; G Siegl; J J Alexander
Journal: Infection Date: 1979 Impact factor: 3.553

7. Induction of hepatitis A virus-neutralizing antibody by a virus-specific synthetic peptide.

Authors: E A Emini; J V Hughes; D S Perlow; J Boger
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8. Molecular cloning and characterization of hepatitis A virus cDNA.

Authors: J R Ticehurst; V R Racaniello; B M Baroudy; D Baltimore; R H Purcell; S M Feinstone
Journal: Proc Natl Acad Sci U S A Date: 1983-10 Impact factor: 11.205

9. Cloning of hepatitis A virus genome.

Authors: K von der Helm; E L Winnacker; F Deinhardt; G Frösner; V Gauss-Müller; B Bayerl; R Scheid; G Siegl
Journal: J Virol Methods Date: 1981-07 Impact factor: 2.014

10. Restricted replication of human hepatitis A virus in cell culture: intracellular biochemical studies.

Authors: S A Locarnini; A G Coulepis; E G Westaway; I D Gust
Journal: J Virol Date: 1981-01 Impact factor: 5.103

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Authors: Ho Yong Chung; Hyun Ho Lee; Kyung Il Kim; Ha Young Chung; Jeon Hwang-Bo; Jong Hwa Park; Garry Sunter; Jong Bum Kim; Dong Hwa Shon; Wonyong Kim; In Sik Chung
Journal: Plant Cell Rep Date: 2011-03-27 Impact factor: 4.570