| Literature DB >> 6267095 |
K von der Helm, E L Winnacker, F Deinhardt, G Frösner, V Gauss-Müller, B Bayerl, R Scheid, G Siegl.
Abstract
Hepatitis A virus (HAV) was highly purified from faeces. The genomic RNA was transcribed to cDNA and this DNA was then cloned into plasmid pBR 322 at the Pst I site, and clones were selected in presence of tetracycline. Most clones contained inserts which hybridized to HAV-specific RNA isolated from HAV-infected cell cultures derived from a human hepatocellular carcinoma. Two clones expressed low amounts of viral antigens.Entities:
Mesh：
Substances：
Year: 1981 PMID： 6267095 DOI： 10.1016/0166-0934(81)90020-3
Source DB: PubMed Journal: J Virol Methods ISSN： 0166-0934 Impact factor: 2.014