Literature DB >> 2817349

A comparison of chemiluminescent and colorimetric substrates in a hepatitis B virus DNA hybridization assay.

I Bronstein1, J C Voyta, B Edwards.   

Abstract

We compared the sensitivity of a chemiluminescent substrate 3-(2'-spiroadamantane)-4-methoxy-4-(3"-phosphoryloxy)phenyl- 1,2-dioxetane (AMPPD) and a chromogenic substrate 5-bromo-4-chloro-3-indolyl phosphate/nitroblue tetrazolium (BCIP/NBT) for detection of an alkaline phosphatase label in a hepatitis B virus "core antigen" DNA (HBVc) probe hybridization assay. Chemiluminescent signal obtained from AMPPD hydrolysis by alkaline phosphatase was detected with Polaroid Instant Black and White Type 612 film. The chemiluminescent assay detected 1.18 x 10(6) copies of HBVc plasmid DNA in 30 min. By comparison, 9.8 x 10(7) copies of DNA could be measured using chromogenic BCIP/NBT substrate within the same incubation time. After further development, the chemiluminescent endpoint permitted detection of 4.39 x 10(4) copies of HBVc plasmid DNA in 2 h.

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Year:  1989        PMID： 2817349     DOI： 10.1016/0003-2697(89)90093-6

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  11 in total

1.  Digital chemiluminescence imaging of DNA sequencing blots using a charge-coupled device camera.

Authors:  A E Karger; R Weiss; R F Gesteland
Journal:  Nucleic Acids Res       Date:  1992-12-25       Impact factor: 16.971

2.  Molecular aspects of cytoplasmic male sterility in perennial ryegrass (Lolium perenne L.): mtDNA and RNA differences between plants with male-sterile and fertile cytoplasm and restriction mapping of their atp6 and coxI homologous regions.

Authors:  G J Rouwendal; J Creemers-Molenaar; F A Krens
Journal:  Theor Appl Genet       Date:  1992-01       Impact factor: 5.699

3.  Chemiluminescence dot blot hybridization assay for detection of B19 parvovirus DNA in human sera.

Authors:  M Musiani; M Zerbini; D Gibellini; G Gentilomi; S Venturoli; G Gallinella; E Ferri; S Girotti
Journal:  J Clin Microbiol       Date:  1991-09       Impact factor: 5.948

4.  Imaging of DNA sequences with chemiluminescence.

Authors:  R Tizard; R L Cate; K L Ramachandran; M Wysk; J C Voyta; O J Murphy; I Bronstein
Journal:  Proc Natl Acad Sci U S A       Date:  1990-06       Impact factor: 11.205

5.  Hybridization and detection of digoxigenin probes on RNA blots.

Authors:  E Davies; R Hodge; P G Isaac
Journal:  Mol Biotechnol       Date:  1996-08       Impact factor: 2.695

6.  Optimization of non-radioactive Southern blot hybridization: single copy detection and reuse of blots.

Authors:  J J Allefs; E M Salentijn; F A Krens; G J Rouwendal
Journal:  Nucleic Acids Res       Date:  1990-05-25       Impact factor: 16.971

7.  Chemiluminescent hybridization assay for hepatitis delta virus RNA in serum.

Authors:  M Gallagher; T T Morris; H A Fields
Journal:  J Clin Microbiol       Date:  1994-04       Impact factor: 5.948

8.  Oligozymes. Oligonucleotide-enzyme conjugates.

Authors:  J L Ruth
Journal:  Mol Biotechnol       Date:  1996-10       Impact factor: 2.695

9.  Detection of Mycoplasma hyopneumoniae by using rRNA-oligodeoxynucleotide hybridization.

Authors:  S Futo; Y Seto; S Mitsuse; Y Mori
Journal:  J Clin Microbiol       Date:  1992-06       Impact factor: 5.948

Review 10.  Interrogating Protein Phosphatases with Chemical Activity Probes.

Authors:  Garrett R Casey; Cliff I Stains
Journal:  Chemistry       Date:  2018-03-08       Impact factor: 5.236
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