Literature DB >> 8887364

Hybridization and detection of digoxigenin probes on RNA blots.

E Davies1, R Hodge, P G Isaac.   

Abstract

This article describes nonradioactive probing of a Northern blot. The method employs digoxigenin-labeled probes. Antidigoxigenin antibody/alkaline phosphate conjugate, and a chemiluminescent substrate are subsequently used in the detection system.

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Year:  1996        PMID： 8887364     DOI： 10.1007/BF02762326

Source DB:  PubMed          Journal:  Mol Biotechnol        ISSN： 1073-6085            Impact factor:   2.695


  3 in total

1.  Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.

Authors:  J C Alwine; D J Kemp; G R Stark
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

2.  A comparison of chemiluminescent and colorimetric substrates in a hepatitis B virus DNA hybridization assay.

Authors:  I Bronstein; J C Voyta; B Edwards
Journal:  Anal Biochem       Date:  1989-07       Impact factor: 3.365

3.  The isolation and characterisation of the tapetum-specific Arabidopsis thaliana A9 gene.

Authors:  W Paul; R Hodge; S Smartt; J Draper; R Scott
Journal:  Plant Mol Biol       Date:  1992-07       Impact factor: 4.076
  3 in total

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