Literature DB >> 1774333

Chemiluminescence dot blot hybridization assay for detection of B19 parvovirus DNA in human sera.

M Musiani1, M Zerbini, D Gibellini, G Gentilomi, S Venturoli, G Gallinella, E Ferri, S Girotti.   

Abstract

A chemiluminescence dot blot hybridization assay was used for the detection of B19 parvovirus DNA in human sera by using digoxigenin-labeled probes. The probes were revealed immunoenzymatically by use of anti-digoxigenin Fab fragments conjugated with alkaline phosphatase. The chemiluminescence signal was obtained by reacting the labeled probe-target complex with an enzyme-triggerable dioxetane substrate. The emitted photons were detected with instant photographic films. In the search for B19 parvovirus DNA, 2,808 serum samples were analyzed.

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Year:  1991        PMID： 1774333      PMCID： PMC270258          DOI： 10.1128/jcm.29.9.2047-2050.1991

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  32 in total

1.  Chemiluminescent assay of alkaline phosphatase applied in an ultrasensitive enzyme immunoassay of thyrotropin.

Authors:  I Bronstein; J C Voyta; G H Thorpe; L J Kricka; G Armstrong
Journal:  Clin Chem       Date:  1989-07       Impact factor: 8.327

2.  Detection of cytomegalovirus DNA using probes labelled with digoxigenin.

Authors:  C P Kimpton; G Corbitt; D J Morris
Journal:  J Virol Methods       Date:  1989-06       Impact factor: 2.014

3.  A hybrido-immunocytochemical assay for the in situ detection of cytomegalovirus DNA using digoxigenin-labeled probes.

Authors:  G Gentilomi; M Musiani; M Zerbini; G Gallinella; D Gibellini; M La Placa
Journal:  J Immunol Methods       Date:  1989-12-20       Impact factor: 2.303

4.  Prolonged parvovirus B19 infection with severe anaemia in a bone marrow transplant patient.

Authors:  H T Weiland; M M Salimans; W E Fibbe; P M Kluin; B J Cohen
Journal:  Br J Haematol       Date:  1989-02       Impact factor: 6.998

5.  Diagnosis of human parvovirus infection by dot-blot hybridization using cloned viral DNA.

Authors:  M J Anderson; S E Jones; A C Minson
Journal:  J Med Virol       Date:  1985-02       Impact factor: 2.327

6.  Chemiluminescent substrates for alkaline phosphatase: application to ultrasensitive enzyme-linked immunoassays and DNA probes.

Authors:  A P Schaap; H Akhavan; L J Romano
Journal:  Clin Chem       Date:  1989-09       Impact factor: 8.327

7.  1,2-dioxetanes: novel chemiluminescent enzyme substrates. Applications to immunoassays.

Authors:  I Bronstein; B Edwards; J C Voyta
Journal:  J Biolumin Chemilumin       Date:  1989-07

8.  A comparison of chemiluminescent and colorimetric substrates in a hepatitis B virus DNA hybridization assay.

Authors:  I Bronstein; J C Voyta; B Edwards
Journal:  Anal Biochem       Date:  1989-07       Impact factor: 3.365

9.  Human parvovirus B19-induced epidemic acute red cell aplasia in patients with hereditary hemolytic anemia.

Authors:  U M Saarinen; T L Chorba; P Tattersall; N S Young; L J Anderson; E Palmer; P F Coccia
Journal:  Blood       Date:  1986-05       Impact factor: 22.113

10.  Dot blot hybridization assay of B19 virus DNA in clinical specimens.

Authors:  J Mori; A M Field; J P Clewley; B J Cohen
Journal:  J Clin Microbiol       Date:  1989-03       Impact factor: 5.948
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  8 in total

1.  Development of a hypersensitive detection method for human parvovirus B19 DNA.

Authors:  K Sato; E Matsuda; K Kamisango; H Iwasaki; S Matsubara; Y Matsunaga
Journal:  J Clin Microbiol       Date:  2000-03       Impact factor: 5.948

2.  Dot immunoperoxidase assay for detection of parvovirus B19 antigens in serum samples.

Authors:  G Gentilomi; M Musiani; M Zerbini; G Gallinella; S Venturoli; E Manaresi
Journal:  J Clin Microbiol       Date:  1997-06       Impact factor: 5.948

3.  Detection of parvovirus B19 DNA in bone marrow cells by chemiluminescence in situ hybridization.

Authors:  M Musiani; A Roda; M Zerbini; G Gentilomi; P Pasini; G Gallinella; S Venturoli
Journal:  J Clin Microbiol       Date:  1996-05       Impact factor: 5.948

4.  Detection of parvovirus B19 in donated blood: a model system for screening by polymerase chain reaction.

Authors:  F McOmish; P L Yap; A Jordan; H Hart; B J Cohen; P Simmonds
Journal:  J Clin Microbiol       Date:  1993-02       Impact factor: 5.948

5.  Chemiluminescent in situ hybridization for the detection of cytomegalovirus DNA.

Authors:  M Musiani; A Roda; M Zerbini; P Pasini; G Gentilomi; G Gallinella; S Venturoli
Journal:  Am J Pathol       Date:  1996-04       Impact factor: 4.307

6.  WIN 54954 treatment of mice infected with a diabetogenic strain of group B coxsackievirus.

Authors:  D M See; J G Tilles
Journal:  Antimicrob Agents Chemother       Date:  1993-08       Impact factor: 5.191

7.  Evaluation of polymerase chain reaction for diagnosis of pneumococcal pneumonia.

Authors:  K M Rudolph; A J Parkinson; C M Black; L W Mayer
Journal:  J Clin Microbiol       Date:  1993-10       Impact factor: 5.948

8.  Different syndromes associated with B19 parvovirus viraemia in paediatric patients: report of four cases.

Authors:  M Zerbini; M Musiani; S Venturoli; G Gallinella; D Gibellini; G Gentilomi; M La Placa
Journal:  Eur J Pediatr       Date:  1992-11       Impact factor: 3.183
  8 in total

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