Literature DB >> 8027326

Chemiluminescent hybridization assay for hepatitis delta virus RNA in serum.

M Gallagher1, T T Morris, H A Fields.   

Abstract

A nonradioactive hybridization assay for the detection of hepatitis delta virus RNA in serum is described. This assay utilizes a digoxigenin-labeled RNA hybridization probe and chemiluminescent immunodetection. The probe can detect as little as 0.4 pg of cDNA with a 60-min exposure. Results obtained are in agreement with serological indicators of hepatitis delta virus infection and are comparable to those obtained by hybridization assays employing radioactively labeled RNA.

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Year:  1994        PMID： 8027326      PMCID： PMC267200          DOI： 10.1128/jcm.32.4.1112-1114.1994

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  9 in total

1.  Comparison of different non-isotopic methods for hepatitis B virus detection in human serum.

Authors:  J M Casacuberta; R Jardi; M Buti; P Puigdoménech; B San Segundo
Journal:  Nucleic Acids Res       Date:  1988-12-23       Impact factor: 16.971

2.  A comparison of chemiluminescent and colorimetric substrates in a hepatitis B virus DNA hybridization assay.

Authors:  I Bronstein; J C Voyta; B Edwards
Journal:  Anal Biochem       Date:  1989-07       Impact factor: 3.365

3.  Identification of two distinct regulatory regions adjacent to the human beta-interferon gene.

Authors:  K Zinn; D DiMaio; T Maniatis
Journal:  Cell       Date:  1983-10       Impact factor: 41.582

4.  Non-radioactive labeling of RNA transcripts in vitro with the hapten digoxigenin (DIG); hybridization and ELISA-based detection.

Authors:  H J Höltke; C Kessler
Journal:  Nucleic Acids Res       Date:  1990-10-11       Impact factor: 16.971

5.  A nonradioactive riboprobe assay for the detection of hepatitis B virus DNA in human sera.

Authors:  A Gerritzen; B Scholt
Journal:  J Virol Methods       Date:  1990-12       Impact factor: 2.014

6.  Molecular cloning and sequencing of a human hepatitis delta (delta) virus RNA.

Authors:  S Makino; M F Chang; C K Shieh; T Kamahora; D M Vannier; S Govindarajan; M M Lai
Journal:  Nature       Date:  1987 Sep 24-30       Impact factor: 49.962

7.  Type D hepatitis: the clinical significance of hepatitis D virus RNA in serum as detected by a hybridization-based assay.

Authors:  A Smedile; M Rizzetto; K Denniston; F Bonino; F Wells; G Verme; F Consolo; B Hoyer; R H Purcell; J L Gerin
Journal:  Hepatology       Date:  1986 Nov-Dec       Impact factor: 17.425

8.  Comparison of radio-labeled DNA probe with a nonisotopic probe for assay of serum hepatitis B virus DNA.

Authors:  D P Molden; R M Nakamura; H Suzuki; S Greer; R G Pergolizzi; C L Brakel
Journal:  Clin Physiol Biochem       Date:  1985

9.  Delta virus infection and severe hepatitis. An epidemic in the Yucpa Indians of Venezuela.

Authors:  S C Hadler; M De Monzon; A Ponzetto; E Anzola; D Rivero; A Mondolfi; A Bracho; D P Francis; M A Gerber; S Thung
Journal:  Ann Intern Med       Date:  1984-03       Impact factor: 25.391
  9 in total

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