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Literature DB >> 27989437

Chromatin Constrains the Initiation and Elongation of DNA Replication.

Sujan Devbhandari¹, Jieqing Jiang¹, Charanya Kumar², Iestyn Whitehouse², Dirk Remus³.

Abstract

Eukaryotic chromosomal DNA is faithfully replicated in a complex series of cell-cycle-regulated events that are incompletely understood. Here we report the reconstitution of DNA replication free in solution with purified proteins from the budding yeast Saccharomyces cerevisiae. The system recapitulates regulated bidirectional origin activation; synthesis of leading and lagging strands by the three replicative DNA polymerases Pol α, Pol δ, and Pol ε; and canonical maturation of Okazaki fragments into continuous daughter strands. We uncover a dual regulatory role for chromatin during DNA replication: promoting origin dependence and determining Okazaki fragment length by restricting Pol δ progression. This system thus provides a functional platform for the detailed mechanistic analysis of eukaryotic chromosome replication.

Entities: Chemical Gene Mutation Species

Keywords: DNA polymerase; DNA replication; McM2-7; ORC; Okazaki fragment; chromatin; lagging strand; leading strand; replication origin

Mesh：

Substances：

Year: 2016 PMID： 27989437 PMCID： PMC5256687 DOI： 10.1016/j.molcel.2016.10.035

Source DB: PubMed Journal: Mol Cell ISSN： 1097-2765 Impact factor: 17.970

37 in total

1. Preparation of nucleosome core particle from recombinant histones.

Authors: K Luger; T J Rechsteiner; T J Richmond
Journal: Methods Enzymol Date: 1999 Impact factor: 1.600

Review 2. DNA replication fidelity.

Authors: Thomas A Kunkel
Journal: J Biol Chem Date: 2004-02-26 Impact factor: 5.157

3. Origin association of Sld3, Sld7, and Cdc45 proteins is a key step for determination of origin-firing timing.

Authors: Seiji Tanaka; Ryuichiro Nakato; Yuki Katou; Katsuhiko Shirahige; Hiroyuki Araki
Journal: Curr Biol Date: 2011-12-08 Impact factor: 10.834

4. MCM2 binding to histones H3-H4 and ASF1 supports a tetramer-to-dimer model for histone inheritance at the replication fork.

Authors: Camille Clément; Geneviève Almouzni
Journal: Nat Struct Mol Biol Date: 2015-08 Impact factor: 15.369

5. Origin plasticity during budding yeast DNA replication in vitro.

Authors: Julien Gros; Sujan Devbhandari; Dirk Remus
Journal: EMBO J Date: 2014-02-24 Impact factor: 11.598

6. Reconsidering DNA Polymerases at the Replication Fork in Eukaryotes.

Authors: Bruce Stillman
Journal: Mol Cell Date: 2015-07-16 Impact factor: 17.970

7. Analysis of the essential functions of the C-terminal protein/protein interaction domain of Saccharomyces cerevisiae pol epsilon and its unexpected ability to support growth in the absence of the DNA polymerase domain.

Authors: R Dua; D L Levy; J L Campbell
Journal: J Biol Chem Date: 1999-08-06 Impact factor: 5.157

8. Intrinsic coupling of lagging-strand synthesis to chromatin assembly.

Authors: Duncan J Smith; Iestyn Whitehouse
Journal: Nature Date: 2012-03-14 Impact factor: 49.962

9. Replication-Coupled Nucleosome Assembly and Positioning by ATP-Dependent Chromatin-Remodeling Enzymes.

Authors: Tejas Yadav; Iestyn Whitehouse
Journal: Cell Rep Date: 2016-04-14 Impact factor: 9.423

10. Reconstitution of a eukaryotic replisome reveals suppression mechanisms that define leading/lagging strand operation.

Authors: Roxana E Georgescu; Grant D Schauer; Nina Y Yao; Lance D Langston; Olga Yurieva; Dan Zhang; Jeff Finkelstein; Mike E O'Donnell
Journal: Elife Date: 2015-04-14 Impact factor: 8.140

61 in total

Chromatin Constrains the Initiation and Elongation of DNA Replication.

1. Preparation of nucleosome core particle from recombinant histones.

Review 2. DNA replication fidelity.

3. Origin association of Sld3, Sld7, and Cdc45 proteins is a key step for determination of origin-firing timing.

4. MCM2 binding to histones H3-H4 and ASF1 supports a tetramer-to-dimer model for histone inheritance at the replication fork.

5. Origin plasticity during budding yeast DNA replication in vitro.

6. Reconsidering DNA Polymerases at the Replication Fork in Eukaryotes.

7. Analysis of the essential functions of the C-terminal protein/protein interaction domain of Saccharomyces cerevisiae pol epsilon and its unexpected ability to support growth in the absence of the DNA polymerase domain.

8. Intrinsic coupling of lagging-strand synthesis to chromatin assembly.

9. Replication-Coupled Nucleosome Assembly and Positioning by ATP-Dependent Chromatin-Remodeling Enzymes.

10. Reconstitution of a eukaryotic replisome reveals suppression mechanisms that define leading/lagging strand operation.

Review 1. The Eukaryotic CMG Helicase at the Replication Fork: Emerging Architecture Reveals an Unexpected Mechanism.

Review 2. The impact of replication stress on replication dynamics and DNA damage in vertebrate cells.

3. Thinking Outside the Cell: Replicating Replication In Vitro.

Review 4. Positive and Negative Regulation of DNA Replication Initiation.

5. Pif1, RPA, and FEN1 modulate the ability of DNA polymerase δ to overcome protein barriers during DNA synthesis.

Review 6. Time for remodeling: SNF2-family DNA translocases in replication fork metabolism and human disease.

Review 7. DNA replication through a chromatin environment.

Review 8. The INO80 remodeller in transcription, replication and repair.

Review 9. Mechanisms of DNA replication termination.

Review 10. The ring-shaped hexameric helicases that function at DNA replication forks.