| Literature DB >> 27980086 |
S John Liu1,2, Max A Horlbeck3,4,5,6, Seung Woo Cho7, Harjus S Birk1,2, Martina Malatesta1,2, Daniel He1,2, Frank J Attenello1,2, Jacqueline E Villalta3,4,5,6, Min Y Cho3,4,5,6, Yuwen Chen3,4,5,6, Mohammad A Mandegar3, Michael P Olvera3, Luke A Gilbert3,4,5,6, Bruce R Conklin3,8,9, Howard Y Chang7, Jonathan S Weissman10,4,5,6, Daniel A Lim11,2,12.
Abstract
The human genome produces thousands of long noncoding RNAs (lncRNAs)-transcripts >200 nucleotides long that do not encode proteins. Although critical roles in normal biology and disease have been revealed for a subset of lncRNAs, the function of the vast majority remains untested. We developed a CRISPR interference (CRISPRi) platform targeting 16,401 lncRNA loci in seven diverse cell lines, including six transformed cell lines and human induced pluripotent stem cells (iPSCs). Large-scale screening identified 499 lncRNA loci required for robust cellular growth, of which 89% showed growth-modifying function exclusively in one cell type. We further found that lncRNA knockdown can perturb complex transcriptional networks in a cell type-specific manner. These data underscore the functional importance and cell type specificity of many lncRNAs.Entities:
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Year: 2016 PMID： 27980086 PMCID： PMC5394926 DOI： 10.1126/science.aah7111
Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728