| Literature DB >> 26317473 |
Hiroshi Nishimasu1, Le Cong2, Winston X Yan3, F Ann Ran4, Bernd Zetsche2, Yinqing Li2, Arisa Kurabayashi5, Ryuichiro Ishitani5, Feng Zhang6, Osamu Nureki7.
Abstract
The RNA-guided DNA endonuclease Cas9 cleaves double-stranded DNA targets with a protospacer adjacent motif (PAM) and complementarity to the guide RNA. Recently, we harnessed Staphylococcus aureus Cas9 (SaCas9), which is significantly smaller than Streptococcus pyogenes Cas9 (SpCas9), to facilitate efficient in vivo genome editing. Here, we report the crystal structures of SaCas9 in complex with a single guide RNA (sgRNA) and its double-stranded DNA targets, containing the 5'-TTGAAT-3' PAM and the 5'-TTGGGT-3' PAM, at 2.6 and 2.7 Å resolutions, respectively. The structures revealed the mechanism of the relaxed recognition of the 5'-NNGRRT-3' PAM by SaCas9. A structural comparison of SaCas9 with SpCas9 highlighted both structural conservation and divergence, explaining their distinct PAM specificities and orthologous sgRNA recognition. Finally, we applied the structural information about this minimal Cas9 to rationally design compact transcriptional activators and inducible nucleases, to further expand the CRISPR-Cas9 genome editing toolbox.Entities:
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Year: 2015 PMID： 26317473 PMCID： PMC4670267 DOI： 10.1016/j.cell.2015.08.007
Source DB: PubMed Journal: Cell ISSN： 0092-8674 Impact factor: 41.582