Literature DB >> 27876939

Molecular detection of Toxoplasma gondii in house sparrow (Passer domesticus) by LAMP and PCR methods in Tehran, Iran.

Amir Abdoli1, Abdolhossein Dalimi1, Haleh Soltanghoraee2, Fatemeh Ghaffarifar1.   

Abstract

Toxoplasma gondii is one of the most common zoonotic parasitic diseases in human and warm-blooded animals worldwide. Birds are one of important intermediate hosts of T. gondii. The aim of this study is molecular detection of T. gondii in the house sparrow by LAMP and PCR methods in Tehran, Iran. A total 200 sparrows were captured in different regions of Tehran. DNA was extracted from tissue samples of each sparrow. LAMP and conventional PCR assays were carried out with a set of primers to detect the 529 bp fragment of T. gondii. LAMP and PCR were detected T. gondii from 17 (8.5 %) and 15 (7.5 %) of 200 sparrows respectively. These results indicated that sensitivity of LAMP was higher than conventional PCR. In our knowledge, this study is the first report of detection of T. gondii by LAMP method in bird hosts. Also, these findings provided an insight into epidemiological pattern of T. gondii infection in sparrow in Iran.

Entities:  

Keywords:  Iran; Loop-mediated isothermal amplification (LAMP); PCR; Sparrow; Toxoplasma gondii

Year:  2015        PMID： 27876939      PMCID： PMC5118306          DOI： 10.1007/s12639-015-0680-2

Source DB:  PubMed          Journal:  J Parasit Dis        ISSN： 0971-7196


  30 in total

1.  Loop-mediated isothermal amplification of DNA.

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Journal:  Nucleic Acids Res       Date:  2000-06-15       Impact factor: 16.971

2.  Loop-mediated isothermal amplification for rapid detection of Bacillus anthracis spores.

Authors:  Yan-Mei Qiao; Yong-Chao Guo; Xian-En Zhang; Ya-Feng Zhou; Zhi-Ping Zhang; Hong-Ping Wei; Rui-Fu Yang; Dian-Bing Wang
Journal:  Biotechnol Lett       Date:  2007-08-03       Impact factor: 2.461

3.  Evaluation of loop-mediated isothermal amplification for detection of Toxoplasma gondii in water samples and comparative findings by polymerase chain reaction and immunofluorescence test (IFT).

Authors:  Isaia Sotiriadou; Panagiotis Karanis
Journal:  Diagn Microbiol Infect Dis       Date:  2008-08-20       Impact factor: 2.803

4.  Development of reverse transcription loop-mediated isothermal amplification (RT-LAMP) as a diagnostic tool of Toxoplasma gondii in pork.

Authors:  Daofeng Qu; Huaiyu Zhou; Jianzhong Han; Siyue Tao; Bailing Zheng; Na Chi; Chunlei Su; Aifang Du
Journal:  Vet Parasitol       Date:  2012-10-23       Impact factor: 2.738

5.  Specific, sensitive, and rapid diagnosis of active toxoplasmosis by a loop-mediated isothermal amplification method using blood samples from patients.

Authors:  Yee Ling Lau; Puviarasi Meganathan; Parthasarathy Sonaimuthu; Girija Thiruvengadam; Veeranoot Nissapatorn; Yeng Chen
Journal:  J Clin Microbiol       Date:  2010-07-21       Impact factor: 5.948

6.  Toxoplasma-safe meat: close to reality?

Authors:  Aize Kijlstra; Erik Jongert
Journal:  Trends Parasitol       Date:  2008-10-24

7.  Detection and genotyping of Toxoplasma gondii strains isolated from birds in the southwest of Iran.

Authors:  S Khademvatan; J Saki; E Yousefi; R Abdizadeh
Journal:  Br Poult Sci       Date:  2013       Impact factor: 2.095

Review 8.  Toxoplasma gondii infections in chickens (Gallus domesticus): prevalence, clinical disease, diagnosis and public health significance.

Authors:  J P Dubey
Journal:  Zoonoses Public Health       Date:  2009-09-10       Impact factor: 2.702

9.  Toxoplasma gondii: sensitive and rapid detection of infection by loop-mediated isothermal amplification (LAMP) method.

Authors:  Houshuang Zhang; Oriel M M Thekisoe; Gabriel O Aboge; Hisako Kyan; Junya Yamagishi; Noboru Inoue; Yoshifumi Nishikawa; Satoshi Zakimi; Xuenan Xuan
Journal:  Exp Parasitol       Date:  2009-02-01       Impact factor: 2.011

10.  Detection of acute toxoplasmosis in pigs using loop-mediated isothermal amplification and quantitative PCR.

Authors:  Yanhua Wang; Guangxiang Wang; Delin Zhang; Hong Yin; Meng Wang
Journal:  Korean J Parasitol       Date:  2013-10-31       Impact factor: 1.341
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  2 in total

1.  CelB is a suitable marker for rapid and specific identification of Klebsiella pneumoniae by the loop-mediated isothermal amplification (LAMP) assay.

Authors:  Yichen Tian; Lefei Wang; Jinyang Zhang; Qinqin Han; Xue-Shan Xia; Yuzhu Song; Guangying Yang
Journal:  Braz J Microbiol       Date:  2019-08-27       Impact factor: 2.476

2.  Molecular Detection and Genotypic Characterization of Toxoplasma gondii in Paraffin-Embedded Fetoplacental Tissues of Women with Recurrent Spontaneous Abortion.

Authors:  Amir Abdoli; Abdolhossein Dalimi; Haleh Soltanghoraee; Fatemeh Ghaffarifar
Journal:  Int J Fertil Steril       Date:  2016-11-01
  2 in total

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