| Literature DB >> 19545521 |
Houshuang Zhang1, Oriel M M Thekisoe, Gabriel O Aboge, Hisako Kyan, Junya Yamagishi, Noboru Inoue, Yoshifumi Nishikawa, Satoshi Zakimi, Xuenan Xuan.
Abstract
Loop-mediated isothermal amplification (LAMP) method amplifies DNA with high specificity, sensitivity and rapidity. In this study, we used a conserved sequence in the 200- to 300-fold repetitive 529 bp gene of Toxoplasma gondii to design primers for LAMP test. Detection limit of T. gondii LAMP assay with the primers is 1 pg/microL of T. gondii DNA, which was evaluated using 10-fold serially diluted DNA of cultured parasites. Furthermore, LAMP and conventional PCR methods were applied for amplification of the T. gondii DNA extracted from the lymph nodes taken from pigs which were suspected to be Toxoplasma infection. As a result, 76.9% (70/91) and 85.7% (78/91) of the samples were positive on PCR and LAMP analyzes, respectively. Therefore, the LAMP has a potential to be applied as an alternative molecular diagnostic tool for detection of T. gondii infection from veterinary samples. This is the first study, which applies the LAMP method to diagnose Toxoplasma from veterinary samples.Entities:
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Year: 2009 PMID： 19545521 DOI： 10.1016/j.exppara.2009.01.012
Source DB: PubMed Journal: Exp Parasitol ISSN： 0014-4894 Impact factor: 2.011