Literature DB >> 17673950

Loop-mediated isothermal amplification for rapid detection of Bacillus anthracis spores.

Yan-Mei Qiao1, Yong-Chao Guo, Xian-En Zhang, Ya-Feng Zhou, Zhi-Ping Zhang, Hong-Ping Wei, Rui-Fu Yang, Dian-Bing Wang.   

Abstract

A loop-mediated isothermal amplification (LAMP) assay system was employed for detecting Bacillus anthracis spores in pure cultures as well as in various simulated powder samples. The specificity of the designed LAMP primer sets was validated by assaying 13 B. anthracis strains and 33 non-B. anthracis species. The detection limits of the LAMP assay were 10 spores/tube for pure cultures and 100 spores/2 mg powder for simulated powder samples. The results show that the LAMP protocol is a promising method for detecting B. anthracis.

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Year:  2007        PMID： 17673950     DOI： 10.1007/s10529-007-9472-9

Source DB:  PubMed          Journal:  Biotechnol Lett        ISSN： 0141-5492            Impact factor:   2.461


  24 in total

1.  Loop-Mediated Isothermal Amplification (LAMP) for Rapid Detection and Quantification of Dehalococcoides Biomarker Genes in Commercial Reductive Dechlorinating Cultures KB-1 and SDC-9.

Authors:  Yogendra H Kanitkar; Robert D Stedtfeld; Robert J Steffan; Syed A Hashsham; Alison M Cupples
Journal:  Appl Environ Microbiol       Date:  2016-01-08       Impact factor: 4.792

2.  The Development of Lyophilized Loop-mediated Isothermal Amplification Reagents for the Detection of Coxiella burnetii.

Authors:  Hua-Wei Chen; Wei-Mei Ching
Journal:  J Vis Exp       Date:  2016-04-18       Impact factor: 1.355

3.  Loop-Mediated Isothermal Amplification for Detection of the 5.8S Ribosomal Ribonucleic Acid Internal Transcribed Spacer 2 Gene Found in Trypanosoma brucei gambiense.

Authors:  Olga V Nikolskaia; Oriel M M Thekisoe; J Stephen Dumler; Dennis J Grab
Journal:  Am J Trop Med Hyg       Date:  2016-06-06       Impact factor: 2.345

4.  Emerging Loop-Mediated Isothermal Amplification-Based Microchip and Microdevice Technologies for Nucleic Acid Detection.

Authors:  Mohammadali Safavieh; Manoj K Kanakasabapathy; Farhang Tarlan; Minhaz U Ahmed; Mohammed Zourob; Waseem Asghar; Hadi Shafiee
Journal:  ACS Biomater Sci Eng       Date:  2016-01-21

5.  Existence of separate domains in lysin PlyG for recognizing Bacillus anthracis spores and vegetative cells.

Authors:  Hang Yang; Dian-Bing Wang; Qiuhua Dong; Zhiping Zhang; Zongqiang Cui; Jiaoyu Deng; Junping Yu; Xian-En Zhang; Hongping Wei
Journal:  Antimicrob Agents Chemother       Date:  2012-07-16       Impact factor: 5.191

6.  Detection of Trypanosoma cruzi and T. rangeli infections from Rhodnius pallescens bugs by loop-mediated isothermal amplification (LAMP).

Authors:  Oriel M M Thekisoe; Carol V Rodriguez; Francisco Rivas; Andrea M Coronel-Servian; Shinya Fukumoto; Chihiro Sugimoto; Shin-Ichiro Kawazu; Noboru Inoue
Journal:  Am J Trop Med Hyg       Date:  2010-05       Impact factor: 2.345

7.  Molecular detection of Toxoplasma gondii in house sparrow (Passer domesticus) by LAMP and PCR methods in Tehran, Iran.

Authors:  Amir Abdoli; Abdolhossein Dalimi; Haleh Soltanghoraee; Fatemeh Ghaffarifar
Journal:  J Parasit Dis       Date:  2015-03-21

8.  Real-time loop-mediated isothermal amplification assay for rapid and sensitive detection of anthrax spores in spiked soil and talcum powder.

Authors:  Neha Jain; Jyoti S Kumar; M M Parida; S Merwyn; G P Rai; G S Agarwal
Journal:  World J Microbiol Biotechnol       Date:  2010-10-21       Impact factor: 3.312

9.  Analytical Performance of a Loop-Mediated Isothermal Amplification Assay for Leishmania DNA Detection in Sandflies and Direct Smears of Patients with Cutaneous Leishmaniasis.

Authors:  Cielo M León; Marina Muñoz; Juan H Tabares; Carolina Hernandez; Carolina Florez; Martha S Ayala; Juan David Ramírez
Journal:  Am J Trop Med Hyg       Date:  2018-03-08       Impact factor: 2.345

10.  Development of a set of three real-time loop-mediated isothermal amplification (LAMP) assays for detection of Bacillus anthracis, the causative agent of anthrax.

Authors:  Swati Banger; Vijai Pal; N K Tripathi; A K Goel
Journal:  Folia Microbiol (Praha)       Date:  2021-04-09       Impact factor: 2.099
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