Tingting Luo1,2, Hongrui Liu2, Wei Feng2, Di Liu2, Juan Du2, Jing Sun2, Wei Wang2, Xiuchun Han2, Jie Guo2, Norio Amizuka3, Xianqi Li1,4, Minqi Li2. 1. School of Stomatology, Shanxi Medical University, Taiyuan, China. 2. Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Department of Bone Metabolism, School of Stomatology Shandong University, Jinan, China. 3. Department of Developmental Biology of Hard Tissue, Graduate School of Dental Medicine, Hokkaido University, Sapporo, Japan. 4. Department of Hard Tissue Research, Graduate School of Oral Medicine, Matsumoto Dental University, Shiojiri, Japan.
Abstract
OBJECTIVES: The purpose of this study was to investigate effects of adipocytes on osteoclast adhesion-related molecules. MATERIALS AND METHODS: ST2 cells, a cloned stromal cell line from mouse bone marrow, able to differentiate into adipocytes, were cultured in serum-free α-MEM which was then collected to be used as adipocyte-conditioned medium (ADIPO CM). RAW264.7 cells were cultured in ADIPO CM in the presence of RANKL, and bone marrow-derived macrophages were cultured in ADIPO CM in the presence of RANKL and macrophage-colony stimulating factor to induce osteoclast differentiation. TRAP staining, resorption pit assay, qRT-PCR and western blotting assays were performed. RESULTS: ELISAs revealed that CXCL12 was abundant in ADIPO CM and CCK-8 assay revealed no proliferation of RAW264.7 cells after exogenous CXCL12 treatment. ADIPO CM enhanced osteoclast formation and resorption, both by RAW264.7 cells and BMMs. In addition, exogenous CXCL12 efficiently potentiated formation of TRAP-positive osteoclast and resorption by RAW264.7 cells. Western blotting and qRT-PCR suggested that ADIPO CM or combined treatment with exogenous CXCL12 caused significant increase in expression of NFAT2, src and osteoclast adhesion-related molecules, including β3 integrin, CD44 and osteopontin. However, these promotional effects were largely abrogated on treatment of AMD3100, a CXCR4 antagonist. CONCLUSIONS: Adipocytes promoted osteoclast differentiation, function and expression of adhesion-related molecules through the CXCL12/CXCR4 signalling pathway.
OBJECTIVES: The purpose of this study was to investigate effects of adipocytes on osteoclast adhesion-related molecules. MATERIALS AND METHODS: ST2 cells, a cloned stromal cell line from mouse bone marrow, able to differentiate into adipocytes, were cultured in serum-free α-MEM which was then collected to be used as adipocyte-conditioned medium (ADIPO CM). RAW264.7 cells were cultured in ADIPO CM in the presence of RANKL, and bone marrow-derived macrophages were cultured in ADIPO CM in the presence of RANKL and macrophage-colony stimulating factor to induce osteoclast differentiation. TRAP staining, resorption pit assay, qRT-PCR and western blotting assays were performed. RESULTS: ELISAs revealed that CXCL12 was abundant in ADIPO CM and CCK-8 assay revealed no proliferation of RAW264.7 cells after exogenous CXCL12 treatment. ADIPO CM enhanced osteoclast formation and resorption, both by RAW264.7 cells and BMMs. In addition, exogenous CXCL12 efficiently potentiated formation of TRAP-positive osteoclast and resorption by RAW264.7 cells. Western blotting and qRT-PCR suggested that ADIPO CM or combined treatment with exogenous CXCL12 caused significant increase in expression of NFAT2, src and osteoclast adhesion-related molecules, including β3 integrin, CD44 and osteopontin. However, these promotional effects were largely abrogated on treatment of AMD3100, a CXCR4 antagonist. CONCLUSIONS: Adipocytes promoted osteoclast differentiation, function and expression of adhesion-related molecules through the CXCL12/CXCR4 signalling pathway.
Authors: Takashi Izawa; Wei Zou; Jean C Chappel; Jason W Ashley; Xu Feng; Steven L Teitelbaum Journal: Mol Cell Biol Date: 2012-05-21 Impact factor: 4.272
Authors: Anne Chabadel; Inmaculada Bañon-Rodríguez; David Cluet; Brian B Rudkin; Bernhard Wehrle-Haller; Elisabeth Genot; Pierre Jurdic; Ines M Anton; Frédéric Saltel Journal: Mol Biol Cell Date: 2007-09-26 Impact factor: 4.138
Authors: Helena B Lopes; Gileade P Freitas; Carlos N Elias; Coralee Tye; Janet L Stein; Gary S Stein; Jane B Lian; Adalberto L Rosa; Marcio M Beloti Journal: J Biomed Mater Res A Date: 2019-02-23 Impact factor: 4.396