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Literature DB >> 27864773

Characterizing Auxin Response Circuits in Saccharomyces cerevisiae by Flow Cytometry.

Edith Pierre-Jerome¹, R Clay Wright¹, Jennifer L Nemhauser².

Abstract

Recapitulation of the nuclear auxin response pathway in Saccharomyces cerevisiae (yeast) provides a means to functionally assay the contribution of individual signaling components to response dynamics. Here, we describe a time course assay for characterizing auxin response circuits using flow cytometry. This method allows for quantitative measurements of the dynamic response of up to 12 circuits (strains) at once. We also describe a steady-state assay and how to utilize an R package we developed to facilitate data analysis.

Entities: Chemical Disease Gene Species

Keywords: Budding yeast; Fluorescent reporters; Signaling dynamics; Synthetic biology

Mesh：

Substances：

Year: 2017 PMID： 27864773 PMCID： PMC5269309 DOI： 10.1007/978-1-4939-6469-7_22

Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745

3 in total

1. Flow cytometric assays for interrogating LAGLIDADG homing endonuclease DNA-binding and cleavage properties.

Authors: Sarah K Baxter; Abigail R Lambert; Andrew M Scharenberg; Jordan Jarjour
Journal: Methods Mol Biol Date: 2013

2. A synthetic approach reveals extensive tunability of auxin signaling.

Authors: Kyle A Havens; Jessica M Guseman; Seunghee S Jang; Edith Pierre-Jerome; Nick Bolten; Eric Klavins; Jennifer L Nemhauser
Journal: Plant Physiol Date: 2012-07-27 Impact factor: 8.340

3. Recapitulation of the forward nuclear auxin response pathway in yeast.

Authors: Edith Pierre-Jerome; Seunghee S Jang; Kyle A Havens; Jennifer L Nemhauser; Eric Klavins
Journal: Proc Natl Acad Sci U S A Date: 2014-06-16 Impact factor: 11.205

3 in total

4 in total

1. Insights into the Evolution and Function of Auxin Signaling F-Box Proteins in Arabidopsis thaliana Through Synthetic Analysis of Natural Variants.

Authors: R Clay Wright; Mollye L Zahler; Stacey R Gerben; Jennifer L Nemhauser
Journal: Genetics Date: 2017-07-31 Impact factor: 4.562

Characterizing Auxin Response Circuits in Saccharomyces cerevisiae by Flow Cytometry.

1. Flow cytometric assays for interrogating LAGLIDADG homing endonuclease DNA-binding and cleavage properties.

2. A synthetic approach reveals extensive tunability of auxin signaling.

3. Recapitulation of the forward nuclear auxin response pathway in yeast.

1. Insights into the Evolution and Function of Auxin Signaling F-Box Proteins in Arabidopsis thaliana Through Synthetic Analysis of Natural Variants.

2. A Synthetic Approach Allows Rapid Characterization of the Maize Nuclear Auxin Response Circuit.

3. A single helix repression domain is functional across diverse eukaryotes.

4. Repression by the Arabidopsis TOPLESS corepressor requires association with the core mediator complex.