Literature DB >> 23423888

Flow cytometric assays for interrogating LAGLIDADG homing endonuclease DNA-binding and cleavage properties.

Sarah K Baxter1, Abigail R Lambert, Andrew M Scharenberg, Jordan Jarjour.   

Abstract

A fast, easy, and scalable method to assess the properties of site-specific nucleases is crucial to -understanding their in cellulo behavior in genome engineering or population-level gene drive applications. Here we describe an analytical platform that enables high-throughput, semiquantitative interrogation of the DNA-binding and catalytic properties of LAGLIDADG homing endonucleases (LHEs). Using this platform, natural or engineered LHEs are expressed on the surface of Saccharomyces cerevisiae yeast where they can be rapidly evaluated against synthetic DNA target sequences using flow cytometry.

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Year:  2013        PMID： 23423888      PMCID： PMC4165528          DOI： 10.1007/978-1-62703-293-3_4

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  16 in total

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Journal:  Cell Mol Life Sci       Date:  1999-08-15       Impact factor: 9.261

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Journal:  FEMS Microbiol Lett       Date:  2000-04-15       Impact factor: 2.742

3.  Frozen competent yeast cells that can be transformed with high efficiency using the LiAc/SS carrier DNA/PEG method.

Authors:  R Daniel Gietz; Robert H Schiestl
Journal:  Nat Protoc       Date:  2007       Impact factor: 13.491

4.  Tapping natural reservoirs of homing endonucleases for targeted gene modification.

Authors:  Ryo Takeuchi; Abigail R Lambert; Amanda Nga-Sze Mak; Kyle Jacoby; Russell J Dickson; Gregory B Gloor; Andrew M Scharenberg; David R Edgell; Barry L Stoddard
Journal:  Proc Natl Acad Sci U S A       Date:  2011-07-22       Impact factor: 11.205

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Journal:  Cell       Date:  1997-05-16       Impact factor: 41.582

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Journal:  Nat Struct Biol       Date:  1997-06

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Journal:  Nucleic Acids Res       Date:  1995-12-25       Impact factor: 16.971

8.  High-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method.

Authors:  R Daniel Gietz; Robert H Schiestl
Journal:  Nat Protoc       Date:  2007       Impact factor: 13.491

9.  Large-scale high-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method.

Authors:  R Daniel Gietz; Robert H Schiestl
Journal:  Nat Protoc       Date:  2007       Impact factor: 13.491

10.  Efficient in toto targeted recombination in mouse liver by meganuclease-induced double-strand break.

Authors:  Agnès Gouble; Julianne Smith; Sylvia Bruneau; Christophe Perez; Valérie Guyot; Jean-Pierre Cabaniols; Sophie Leduc; Laurence Fiette; Patrick Avé; Béatrice Micheau; Philippe Duchateau; Frédéric Pâques
Journal:  J Gene Med       Date:  2006-05       Impact factor: 4.565
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  9 in total

1.  Optimization of Protein Thermostability and Exploitation of Recognition Behavior to Engineer Altered Protein-DNA Recognition.

Authors:  Abigail R Lambert; Jazmine P Hallinan; Rachel Werther; Dawid Głów; Barry L Stoddard
Journal:  Structure       Date:  2020-04-30       Impact factor: 5.006

Review 2.  Gene editing and its application for hematological diseases.

Authors:  Mark J Osborn; Joseph J Belanto; Jakub Tolar; Daniel F Voytas
Journal:  Int J Hematol       Date:  2016-05-27       Impact factor: 2.490

3.  Tuning DNA binding affinity and cleavage specificity of an engineered gene-targeting nuclease via surface display, flow cytometry and cellular analyses.

Authors:  Nixon Niyonzima; Abigail R Lambert; Rachel Werther; Harshana De Silva Feelixge; Pavitra Roychoudhury; Alexander L Greninger; Daniel Stone; Barry L Stoddard; Keith R Jerome
Journal:  Protein Eng Des Sel       Date:  2017-07-01       Impact factor: 1.650

4.  Characterizing Auxin Response Circuits in Saccharomyces cerevisiae by Flow Cytometry.

Authors:  Edith Pierre-Jerome; R Clay Wright; Jennifer L Nemhauser
Journal:  Methods Mol Biol       Date:  2017

5.  The Structural Basis of Asymmetry in DNA Binding and Cleavage as Exhibited by the I-SmaMI LAGLIDADG Meganuclease.

Authors:  Betty W Shen; Abigail Lambert; Bradley C Walker; Barry L Stoddard; Brett K Kaiser
Journal:  J Mol Biol       Date:  2015-12-15       Impact factor: 5.469

6.  Crystallographic analyses illustrate significant plasticity and efficient recoding of meganuclease target specificity.

Authors:  Rachel Werther; Jazmine P Hallinan; Abigail R Lambert; Kyle Havens; Mark Pogson; Jordan Jarjour; Roberto Galizi; Nikolai Windbichler; Andrea Crisanti; Tony Nolan; Barry L Stoddard
Journal:  Nucleic Acids Res       Date:  2017-08-21       Impact factor: 16.971

Review 7.  Heritable strategies for controlling insect vectors of disease.

Authors:  Austin Burt
Journal:  Philos Trans R Soc Lond B Biol Sci       Date:  2014-05-12       Impact factor: 6.237

8.  Digital detection of endonuclease mediated gene disruption in the HIV provirus.

Authors:  Ruth Hall Sedlak; Shu Liang; Nixon Niyonzima; Harshana S De Silva Feelixge; Pavitra Roychoudhury; Alexander L Greninger; Nicholas D Weber; Sandrine Boissel; Andrew M Scharenberg; Anqi Cheng; Amalia Magaret; Roger Bumgarner; Daniel Stone; Keith R Jerome
Journal:  Sci Rep       Date:  2016-02-02       Impact factor: 4.379

Review 9.  Engineering altered protein-DNA recognition specificity.

Authors:  Adam J Bogdanove; Andrew Bohm; Jeffrey C Miller; Richard D Morgan; Barry L Stoddard
Journal:  Nucleic Acids Res       Date:  2018-06-01       Impact factor: 16.971
  9 in total

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