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Literature DB >> 27798609

Simultaneous dual-color fluorescence lifetime imaging with novel red-shifted fluorescent proteins.

Tal Laviv¹, Benjamin B Kim², Jun Chu^2,3, Amy J Lam^2,3, Michael Z Lin^2,3,4, Ryohei Yasuda¹.

Abstract

We describe a red-shifted fluorescence resonance energy transfer (FRET) pair optimized for dual-color fluorescence lifetime imaging (FLIM). This pair utilizes a newly developed FRET donor, monomeric cyan-excitable red fluorescent protein (mCyRFP1), which has a large Stokes shift and a monoexponential fluorescence lifetime decay. When used together with EGFP-based biosensors, the new pair enables simultaneous imaging of the activities of two signaling molecules in single dendritic spines undergoing structural plasticity.

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Year: 2016 PMID： 27798609 PMCID： PMC5322478 DOI： 10.1038/nmeth.4046

Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547

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Review 3. A guide to choosing fluorescent proteins.

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4. Highly sensitive and quantitative FRET-FLIM imaging in single dendritic spines using improved non-radiative YFP.

Authors: Hideji Murakoshi; Seok-Jin Lee; Ryohei Yasuda
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Journal: Nat Methods Date: 2016-05-30 Impact factor: 28.547

8. A bright cyan-excitable orange fluorescent protein facilitates dual-emission microscopy and enhances bioluminescence imaging in vivo.

Authors: Jun Chu; Younghee Oh; Alex Sens; Niloufar Ataie; Hod Dana; John J Macklin; Tal Laviv; Erik S Welf; Kevin M Dean; Feijie Zhang; Benjamin B Kim; Clement Tran Tang; Michelle Hu; Michelle A Baird; Michael W Davidson; Mark A Kay; Reto Fiolka; Ryohei Yasuda; Douglas S Kim; Ho-Leung Ng; Michael Z Lin
Journal: Nat Biotechnol Date: 2016-05-30 Impact factor: 54.908

9. Activation of CaMKII in single dendritic spines during long-term potentiation.

Authors: Seok-Jin R Lee; Yasmin Escobedo-Lozoya; Erzsebet M Szatmari; Ryohei Yasuda
Journal: Nature Date: 2009-03-19 Impact factor: 49.962

10. Ultrasensitive fluorescent proteins for imaging neuronal activity.

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Review 6. Biophysics of Biochemical Signaling in Dendritic Spines: Implications in Synaptic Plasticity.

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