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Literature DB >> 277917

In vitro biosynthesis and chemical characterization of beta-lipotropin, gamma-lipotropin, and beta-endorphin in rat pars intermedia.

N G Seidah, C Gianoulakis, P Crine, M Lis, S Benjannet, R Routhier, M Chrétien.

Abstract

Three 3-hr incubations of pars intermedia cells from 40 rat pituitaries with [35S]methionine, [3H]lysine, and [3H]leucine sufficed for the identification and chemical characterization of biosynthesized beta-lipotropin, gamma-lipotropin, and beta-endorphin. From the molecular weight, migration on polyacrylamide gels, and sequence Met5, Lys9, Leu14,17, rat beta-endorphin was shown to be identical to its sheep homologue and no trace of Leu5 beta-endorphin could be detected. Rat beta-lipotropin differs from that of sheep in its elution properties on CM-cellulose, and its sequence shows Leu2,10,14, Lys20. Rat gamma-lipotropin shows the same NH2-terminal sequence as beta-lipotropin and is again different from its sheep homologue. The identification of rat beta-lipotropin was confirmed by its selective cleavage into beta-endorphin after trypsin digestion of the citraconylated peptide, a property not observed with rat gamma-lipotropin.

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Year: 1978 PMID： 277917 PMCID： PMC392732 DOI： 10.1073/pnas.75.7.3153

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

19 in total

1. Disk electrophoresis of basic proteins and peptides on polyacrylamide gels.

Authors: R A REISFELD; U J LEWIS; D E WILLIAMS
Journal: Nature Date: 1962-07-21 Impact factor: 49.962

2. beta-Endorphin: a pituitary peptide with potent morphine-like activity.

Authors: C H Li
Journal: Arch Biochem Biophys Date: 1977-10 Impact factor: 4.013

3. Isolation and primary structure of beta-endrophin and beta-lipotropin from bovine pituitary glands.

Authors: C H Li; L Tan; D Chung
Journal: Biochem Biophys Res Commun Date: 1977-08-08 Impact factor: 3.575

4. Isolation and characterization of the opioid peptides from rat pituitary: beta-endorphin.

Authors: M Rubinstein; S Stein; S Udenfriend
Journal: Proc Natl Acad Sci U S A Date: 1977-11 Impact factor: 11.205

5. In vitro biosynthesis of beta-endorphin, gamma-lipoprotein, and beta-lipotropin by the pars intermedia of beef pituitary glands.

Authors: P Crine; S Benjannet; N G Seidah; M Lis; M Chrétien
Journal: Proc Natl Acad Sci U S A Date: 1977-10 Impact factor: 11.205

6. Lipotropin: localization by radioimmunoassay of endorphin precursors in pituitary and brain.

Authors: F LaBella; G Queen; J Senyshyn; M Lis; M Chretien
Journal: Biochem Biophys Res Commun Date: 1977-03-21 Impact factor: 3.575

7. Common precursor to corticotropins and endorphins.

Authors: R E Mains; B A Eipper; N Ling
Journal: Proc Natl Acad Sci U S A Date: 1977-07 Impact factor: 11.205

8. Isolation and characterization of the opioid peptides from rat pituitary: beta-lipotropin.

Authors: M Rubinstein; S Stein; L D Gerber; S Udenfriend
Journal: Proc Natl Acad Sci U S A Date: 1977-07 Impact factor: 11.205

9. Immunohistochemical localization of beta-lipotropic hormone in the pituitary gland.

Authors: G Pelletier; R Leclerc; F Labrie; J Cote; M Chretien; M Lis
Journal: Endocrinology Date: 1977-03 Impact factor: 4.736

10. In vitro biosynthesis of beta-endorphin in pituitary glands.

Authors: P Crine; S Benjannet; N G Seidah; M Lis; M Chrétien
Journal: Proc Natl Acad Sci U S A Date: 1977-04 Impact factor: 11.205

10 in total

1. Concomitant synthesis of beta-endorphin and alpha-melanotropin from two forms of pro-opiomelanocortin in the rat pars intermedia.

Authors: P Crine; F Gossard; N G Seidah; L Blanchette; M Lis; M Chrétien
Journal: Proc Natl Acad Sci U S A Date: 1979-10 Impact factor: 11.205

9. Biosynthesis of precursor corticotropin/endorphin-, corticotropin-, alpha-melanotropin-, beta-lipotropin-, and beta-endorphin-like material by cultured neonatal rat hypothalamic neurons.

Authors: A S Liotta; C Loudes; J F McKelvy; D T Krieger
Journal: Proc Natl Acad Sci U S A Date: 1980-04 Impact factor: 11.205

Review 10. Chemistry and biosynthesis of pro-opiomelanocortin. ACTH, MSH's, endorphins and their related peptides.

Authors: M Chrétien; N G Seidah
Journal: Mol Cell Biochem Date: 1981-01-28 Impact factor: 3.396

10 in total

In vitro biosynthesis and chemical characterization of beta-lipotropin, gamma-lipotropin, and beta-endorphin in rat pars intermedia.

1. Disk electrophoresis of basic proteins and peptides on polyacrylamide gels.

2. beta-Endorphin: a pituitary peptide with potent morphine-like activity.

3. Isolation and primary structure of beta-endrophin and beta-lipotropin from bovine pituitary glands.

4. Isolation and characterization of the opioid peptides from rat pituitary: beta-endorphin.

5. In vitro biosynthesis of beta-endorphin, gamma-lipoprotein, and beta-lipotropin by the pars intermedia of beef pituitary glands.

6. Lipotropin: localization by radioimmunoassay of endorphin precursors in pituitary and brain.

7. Common precursor to corticotropins and endorphins.

8. Isolation and characterization of the opioid peptides from rat pituitary: beta-lipotropin.

9. Immunohistochemical localization of beta-lipotropic hormone in the pituitary gland.

10. In vitro biosynthesis of beta-endorphin in pituitary glands.

1. Concomitant synthesis of beta-endorphin and alpha-melanotropin from two forms of pro-opiomelanocortin in the rat pars intermedia.

2. Biosynthesis of beta-endorphin from beta-lipotropin and a larger molecular weight precursor in rat pars intermedia.

3. Localization of corticotropin- and endorphin-related peptides in the intermediate lobe of the rat pituitary.

4. Action of cathepsin D on human beta-lipotropin: a possible source of human "beta-melanotropin".

5. Distribution of active and inactive forms of endorphins in rat pituitary and brain.

6. Biosynthesis of hormonal and neural peptides.

7. Neurons of the rat hypothalamus reactive with antisera against endorphins, ACTH, MSH and beta-LPH.

8. PC1 and PC2 are proprotein convertases capable of cleaving proopiomelanocortin at distinct pairs of basic residues.

9. Biosynthesis of precursor corticotropin/endorphin-, corticotropin-, alpha-melanotropin-, beta-lipotropin-, and beta-endorphin-like material by cultured neonatal rat hypothalamic neurons.

Review 10. Chemistry and biosynthesis of pro-opiomelanocortin. ACTH, MSH's, endorphins and their related peptides.