| Literature DB >> 27749839 |
Christine Koehler1, Paul F Sauter2, Mirella Wawryszyn2, Gemma Estrada Girona1, Kapil Gupta3, Jonathan J M Landry1, Markus Hsi-Yang Fritz1, Ksenija Radic1, Jan-Erik Hoffmann1, Zhuo A Chen4, Juan Zou4, Piau Siong Tan1, Bence Galik5, Sini Junttila5, Peggy Stolt-Bergner5, Giancarlo Pruneri6, Attila Gyenesei5, Carsten Schultz1, Moritz Bosse Biskup2, Hueseyin Besir1, Vladimir Benes1, Juri Rappsilber4,7, Martin Jechlinger1, Jan O Korbel1, Imre Berger3,8, Stefan Braese2,9, Edward A Lemke1.
Abstract
We present a baculovirus-based protein engineering method that enables site-specific introduction of unique functionalities in a eukaryotic protein complex recombinantly produced in insect cells. We demonstrate the versatility of this efficient and robust protein production platform, 'MultiBacTAG', (i) for the fluorescent labeling of target proteins and biologics using click chemistries, (ii) for glycoengineering of antibodies, and (iii) for structure-function studies of novel eukaryotic complexes using single-molecule Förster resonance energy transfer as well as site-specific crosslinking strategies.Mesh：
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Year: 2016 PMID： 27749839 DOI： 10.1038/nmeth.4032
Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547