Literature DB >> 2762122

Chemiluminescent detection of DNA: application for DNA sequencing and hybridization.

S Beck1, T O'Keeffe, J M Coull, H Köster.   

Abstract

A non-radioactive DNA detection chemistry is described and its application is shown for DNA hybridization and standard dideoxy DNA sequencing. The method employes a biotin-streptavidin system which binds an enzyme specifically to a target DNA and upon exposure to substrate, the enzyme catalyzes a chemiluminescent reaction. The image is captured within seconds by a Polaroid or X-ray film. The method is capable of detecting DNA in the hundred attomol range.

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Year:  1989        PMID： 2762122      PMCID： PMC318099          DOI： 10.1093/nar/17.13.5115

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  22 in total

1.  DNA sequencing with direct blotting electrophoresis and colorimetric detection.

Authors:  P Richterich; C Heller; H Wurst; F M Pohl
Journal:  Biotechniques       Date:  1989-01       Impact factor: 1.993

2.  A new method for sequencing DNA.

Authors:  A M Maxam; W Gilbert
Journal:  Proc Natl Acad Sci U S A       Date:  1977-02       Impact factor: 11.205

3.  Automatic reading of DNA sequencing gel autoradiographs using a large format digital scanner.

Authors:  J K Elder; D K Green; E M Southern
Journal:  Nucleic Acids Res       Date:  1986-01-10       Impact factor: 16.971

4.  Efficient methods for attaching non-radioactive labels to the 5' ends of synthetic oligodeoxyribonucleotides.

Authors:  S Agrawal; C Christodoulou; M J Gait
Journal:  Nucleic Acids Res       Date:  1986-08-11       Impact factor: 16.971

5.  Luminescent detection method for immunodot, Western, and Southern blots.

Authors:  M M Leong; C Milstein; R Pannell
Journal:  J Histochem Cytochem       Date:  1986-12       Impact factor: 2.479

6.  Genomic sequencing.

Authors:  G M Church; W Gilbert
Journal:  Proc Natl Acad Sci U S A       Date:  1984-04       Impact factor: 11.205

7.  The synthesis of oligonucleotides containing an aliphatic amino group at the 5' terminus: synthesis of fluorescent DNA primers for use in DNA sequence analysis.

Authors:  L M Smith; S Fung; M W Hunkapiller; T J Hunkapiller; L E Hood
Journal:  Nucleic Acids Res       Date:  1985-04-11       Impact factor: 16.971

8.  Polymer support oligonucleotide synthesis XVIII: use of beta-cyanoethyl-N,N-dialkylamino-/N-morpholino phosphoramidite of deoxynucleosides for the synthesis of DNA fragments simplifying deprotection and isolation of the final product.

Authors:  N D Sinha; J Biernat; J McManus; H Köster
Journal:  Nucleic Acids Res       Date:  1984-06-11       Impact factor: 16.971

9.  A simple and efficient procedure for the synthesis of 5'-aminoalkyl oligodeoxynucleotides.

Authors:  L Wachter; J A Jablonski; K L Ramachandran
Journal:  Nucleic Acids Res       Date:  1986-10-24       Impact factor: 16.971

10.  DNA sequencing with direct blotting electrophoresis.

Authors:  S Beck; F M Pohl
Journal:  EMBO J       Date:  1984-12-01       Impact factor: 11.598
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  12 in total

1.  Digital chemiluminescence imaging of DNA sequencing blots using a charge-coupled device camera.

Authors:  A E Karger; R Weiss; R F Gesteland
Journal:  Nucleic Acids Res       Date:  1992-12-25       Impact factor: 16.971

2.  Enzymatic multiplex DNA sequencing.

Authors:  M Chee
Journal:  Nucleic Acids Res       Date:  1991-06-25       Impact factor: 16.971

3.  Mutant profiles of selectable genetic elements.

Authors:  H Wurst; F M Pohl
Journal:  Proc Natl Acad Sci U S A       Date:  1991-11-15       Impact factor: 11.205

4.  Imaging of DNA sequences with chemiluminescence.

Authors:  R Tizard; R L Cate; K L Ramachandran; M Wysk; J C Voyta; O J Murphy; I Bronstein
Journal:  Proc Natl Acad Sci U S A       Date:  1990-06       Impact factor: 11.205

5.  Optimization of non-radioactive Southern blot hybridization: single copy detection and reuse of blots.

Authors:  J J Allefs; E M Salentijn; F A Krens; G J Rouwendal
Journal:  Nucleic Acids Res       Date:  1990-05-25       Impact factor: 16.971

6.  Clinical performance of non-radioactive assays for HIV-1 DNA amplified by the polymerase chain reaction.

Authors:  J M Wages; M Hamdallah; A K Fowler; C N Roberts; R R Redfield; D S Burke
Journal:  World J Microbiol Biotechnol       Date:  1993-01       Impact factor: 3.312

Review 7.  DNA sequencing. Recent innovations and future trends.

Authors:  H G Griffin; A M Griffin
Journal:  Appl Biochem Biotechnol       Date:  1993 Jan-Feb       Impact factor: 2.926

8.  Non-radioactive labeling of RNA transcripts in vitro with the hapten digoxigenin (DIG); hybridization and ELISA-based detection.

Authors:  H J Höltke; C Kessler
Journal:  Nucleic Acids Res       Date:  1990-10-11       Impact factor: 16.971

9.  Antibacterial activity of gatifloxacin (AM-1155, CG5501, BMS-206584), a newly developed fluoroquinolone, against sequentially acquired quinolone-resistant mutants and the norA transformant of Staphylococcus aureus.

Authors:  H Fukuda; S Hori; K Hiramatsu
Journal:  Antimicrob Agents Chemother       Date:  1998-08       Impact factor: 5.191

10.  Primary targets of fluoroquinolones in Streptococcus pneumoniae.

Authors:  H Fukuda; K Hiramatsu
Journal:  Antimicrob Agents Chemother       Date:  1999-02       Impact factor: 5.191
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