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Literature DB >> 2216776

Non-radioactive labeling of RNA transcripts in vitro with the hapten digoxigenin (DIG); hybridization and ELISA-based detection.

Abstract

We have developed a system for the enzymatic in vitro synthesis of non-radioactively labeled RNA which is derivatized with the hapten digoxigenin (DIG). The labeling reaction as well as the conditions for hybridization and detection of hybrids by an antibody-conjugate and a coupled colour reaction were analyzed and adapted for high sensitivity and low background. In addition, data on the performance and sensitivity of digoxigenin-labeled RNA probes in Southern and Northern blots are presented.

Entities: Chemical

Mesh：

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Year: 1990 PMID： 2216776 PMCID： PMC332324 DOI： 10.1093/nar/18.19.5843

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

28 in total

1. Non-radioactive hybridization probes prepared by the chemical labelling of DNA and RNA with a novel reagent, photobiotin.

Authors: A C Forster; J L McInnes; D C Skingle; R H Symons
Journal: Nucleic Acids Res Date: 1985-02-11 Impact factor: 16.971

2. Injected anti-sense RNAs specifically block messenger RNA translation in vivo.

Authors: D A Melton
Journal: Proc Natl Acad Sci U S A Date: 1985-01 Impact factor: 11.205

3. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.

Authors: D A Melton; P A Krieg; M R Rebagliati; T Maniatis; K Zinn; M R Green
Journal: Nucleic Acids Res Date: 1984-09-25 Impact factor: 16.971

4. Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter.

Authors: P J Southern; P Berg
Journal: J Mol Appl Genet Date: 1982

Review 5. Enzyme-labeling of antibodies and their fragments for enzyme immunoassay and immunohistochemical staining.

Authors: E Ishikawa; M Imagawa; S Hashida; S Yoshitake; Y Hamaguchi; T Ueno
Journal: J Immunoassay Date: 1983

6. Evolutionary conservation in the untranslated regions of actin mRNAs: DNA sequence of a human beta-actin cDNA.

Authors: P Ponte; S Y Ng; J Engel; P Gunning; L Kedes
Journal: Nucleic Acids Res Date: 1984-02-10 Impact factor: 16.971

7. Detection of mrnas in sea urchin embryos by in situ hybridization using asymmetric RNA probes.

Authors: K H Cox; D V DeLeon; L M Angerer; R C Angerer
Journal: Dev Biol Date: 1984-02 Impact factor: 3.582

8. Purification of human and bovine alkaline phosphatases by affinity chromatography.

Authors: E Mössner; M Boll; G Pfleiderer
Journal: Hoppe Seylers Z Physiol Chem Date: 1980-04

9. High-resolution 1H nuclear magnetic resonance study of cerebral hypoxia in vivo.

Authors: K L Behar; J A den Hollander; M E Stromski; T Ogino; R G Shulman; O A Petroff; J W Prichard
Journal: Proc Natl Acad Sci U S A Date: 1983-08 Impact factor: 11.205

10. Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes.

Authors: P R Langer; A A Waldrop; D C Ward
Journal: Proc Natl Acad Sci U S A Date: 1981-11 Impact factor: 11.205

39 in total

1. Stimulation of GABAB receptors increases the expression of the proenkephalin gene in slice cultures of rat neocortex.

Authors: F Mörl; J Leemhuis; K Lindemeyer; N Grass; W Nörenberg; D K Meyer
Journal: Naunyn Schmiedebergs Arch Pharmacol Date: 2003-05-06 Impact factor: 3.000

2. Detection of mRNA in streptomyces cells by whole-cell hybridization with digoxigenin-labeled probes.

Authors: D Hahn; R I Amann; J Zeyer
Journal: Appl Environ Microbiol Date: 1993-08 Impact factor: 4.792

3. Identification of Whole Fixed Bacterial Cells with Nonradioactive 23S rRNA-Targeted Polynucleotide Probes.

Authors: K Trebesius; R Amann; W Ludwig; K Mühlegger; K H Schleifer
Journal: Appl Environ Microbiol Date: 1994-09 Impact factor: 4.792

4. Expression cloning of the Golgi CMP-sialic acid transporter.

Authors: M Eckhardt; M Mühlenhoff; A Bethe; R Gerardy-Schahn
Journal: Proc Natl Acad Sci U S A Date: 1996-07-23 Impact factor: 11.205

5. Regulation of the expression of the proenkephalin gene in cultured meningeal fibroblasts: opposite effects of alpha 1- and beta 2-adrenoceptors.

Authors: B Hildebrand; B Wissler; C Olenik; D K Meyer
Journal: Naunyn Schmiedebergs Arch Pharmacol Date: 1996-10 Impact factor: 3.000

Review 6. RNA molecules lighting up under the microscope.

Authors: R W Dirks
Journal: Histochem Cell Biol Date: 1996-08 Impact factor: 4.304

Review 7. Cytochemical detection systems for in situ hybridization, and the combination with immunocytochemistry, 'who is still afraid of red, green and blue?'.

Authors: E J Speel; F C Ramaekers; A H Hopman
Journal: Histochem J Date: 1995-11