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Literature DB >> 3753777

Automatic reading of DNA sequencing gel autoradiographs using a large format digital scanner.

Abstract

We have developed a large format digital scanner for several applications in nucleic acid analysis. Here we describe the scanning of autoradiographs of DNA sequencing gels and a set of programs for reading the base sequence. The programs correct distortions in the gel, recognize bands by their characteristic shape and assign bases to bands by weighting band position and intensity. The sequence read in this way is as accurate as that read by an expert.

Mesh：

Substances：
DNA

Year: 1986 PMID： 3753777 PMCID： PMC339425 DOI： 10.1093/nar/14.1.417

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

4 in total

1. An inexpensive semi-automated sequence reader for the Apple II computer.

Authors: M Komaromy; H Govan
Journal: Nucleic Acids Res Date: 1984-01-11 Impact factor: 16.971

2. A computer program to enter DNA gel reading data into a computer.

Authors: R Staden
Journal: Nucleic Acids Res Date: 1984-01-11 Impact factor: 16.971

3. Computer-based image analysis of one-dimensional electrophoretic gels used for the separation of DNA restriction fragments.

Authors: A J Gray; D E Beecher; M V Olson
Journal: Nucleic Acids Res Date: 1984-01-11 Impact factor: 16.971

4. Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination.

Authors: M D Biggin; T J Gibson; G F Hong
Journal: Proc Natl Acad Sci U S A Date: 1983-07 Impact factor: 11.205

4 in total

13 in total

10. Genomic organization of the human asparagine transfer RNA genes: localization to the U1 RNA gene and class I pseudogene repeat units.

Authors: R A Buckland
Journal: Am J Hum Genet Date: 1989-08 Impact factor: 11.025

Automatic reading of DNA sequencing gel autoradiographs using a large format digital scanner.

1. An inexpensive semi-automated sequence reader for the Apple II computer.

2. A computer program to enter DNA gel reading data into a computer.

3. Computer-based image analysis of one-dimensional electrophoretic gels used for the separation of DNA restriction fragments.

4. Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination.

1. Distribution of Alu and L1 repeats in human YAC recombinants.

2. Digital chemiluminescence imaging of DNA sequencing blots using a charge-coupled device camera.

3. Enzymatic multiplex DNA sequencing.

4. Imaging of DNA sequences with chemiluminescence.

5. Automated sequence reading and analysis.

6. Solid phase DNA sequencing using the biotin-avidin system.

7. Chemiluminescent detection of DNA: application for DNA sequencing and hybridization.

8. Sequence errors described in GenBank: a means to determine the accuracy of DNA sequence interpretation.

9. Human telomeres contain at least three types of G-rich repeat distributed non-randomly.

10. Genomic organization of the human asparagine transfer RNA genes: localization to the U1 RNA gene and class I pseudogene repeat units.