Ping Li1, Xuan Li1,2, Qing Gu1, Xiu-Yu Lou1, Xiao-Mei Zhang1, Da-Feng Song1, Chen Zhang1. 1. Key Laboratory for Food Microbial Technology of Zhejiang Province, College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, China. 2. College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China.
Abstract
OBJECTIVE: In previous studies, Lactobacillus plantarum ZJ316 showed probiotic properties, such as antimicrobial activity against various pathogens and the capacity to significantly improve pig growth and pork quality. The purpose of this study was to reveal the genes potentially related to its genetic adaptation and probiotic profiles based on comparative genomic analysis. METHODS: The genome sequence of L. plantarum ZJ316 was compared with those of eight L. plantarum strains deposited in GenBank. BLASTN, Mauve, and MUMmer programs were used for genome alignment and comparison. CRISPRFinder was applied for searching the clustered regularly interspaced short palindromic repeats (CRISPRs). RESULTS: We identified genes that encode proteins related to genetic adaptation and probiotic profiles, including carbohydrate transport and metabolism, proteolytic enzyme systems and amino acid biosynthesis, CRISPR adaptive immunity, stress responses, bile salt resistance, ability to adhere to the host intestinal wall, exopolysaccharide (EPS) biosynthesis, and bacteriocin biosynthesis. CONCLUSIONS: Comparative characterization of the L. plantarum ZJ316 genome provided the genetic basis for further elucidating the functional mechanisms of its probiotic properties. ZJ316 could be considered a potential probiotic candidate.
OBJECTIVE: In previous studies, Lactobacillus plantarum ZJ316 showed probiotic properties, such as antimicrobial activity against various pathogens and the capacity to significantly improve pig growth and pork quality. The purpose of this study was to reveal the genes potentially related to its genetic adaptation and probiotic profiles based on comparative genomic analysis. METHODS: The genome sequence of L. plantarum ZJ316 was compared with those of eight L. plantarum strains deposited in GenBank. BLASTN, Mauve, and MUMmer programs were used for genome alignment and comparison. CRISPRFinder was applied for searching the clustered regularly interspaced short palindromic repeats (CRISPRs). RESULTS: We identified genes that encode proteins related to genetic adaptation and probiotic profiles, including carbohydrate transport and metabolism, proteolytic enzyme systems and amino acid biosynthesis, CRISPR adaptive immunity, stress responses, bile salt resistance, ability to adhere to the host intestinal wall, exopolysaccharide (EPS) biosynthesis, and bacteriocin biosynthesis. CONCLUSIONS: Comparative characterization of the L. plantarum ZJ316 genome provided the genetic basis for further elucidating the functional mechanisms of its probiotic properties. ZJ316 could be considered a potential probiotic candidate.
Entities:
Keywords:
Adaptation; Comparative genomics; Lactobacillus plantarum ZJ316; Probiotics
Authors: Colin Hill; Francisco Guarner; Gregor Reid; Glenn R Gibson; Daniel J Merenstein; Bruno Pot; Lorenzo Morelli; Roberto Berni Canani; Harry J Flint; Seppo Salminen; Philip C Calder; Mary Ellen Sanders Journal: Nat Rev Gastroenterol Hepatol Date: 2014-06-10 Impact factor: 46.802
Authors: Guanghua Zhao; Pierpaolo Ceci; Andrea Ilari; Laura Giangiacomo; Thomas M Laue; Emilia Chiancone; N Dennis Chasteen Journal: J Biol Chem Date: 2002-05-16 Impact factor: 5.157
Authors: Lars Axelsson; Ida Rud; Kristine Naterstad; Hans Blom; Bernadet Renckens; Jos Boekhorst; Michiel Kleerebezem; Sacha van Hijum; Roland J Siezen Journal: J Bacteriol Date: 2012-05 Impact factor: 3.490
Authors: Roland J Siezen; Vesela A Tzeneva; Anna Castioni; Michiel Wels; Hoa T K Phan; Jan L W Rademaker; Marjo J C Starrenburg; Michiel Kleerebezem; Douwe Molenaar; Johan E T van Hylckama Vlieg Journal: Environ Microbiol Date: 2009-12-04 Impact factor: 5.491
Authors: Paul Benedic U Salvador; Leslie Michelle M Dalmacio; Sang Hoon Kim; Dae-Kyung Kang; Marilen P Balolong Journal: Access Microbiol Date: 2021-12-17