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Literature DB >> 2747635

Isolation of a cDNA clone for human threonyl-tRNA synthetase: amplification of the structural gene in borrelidin-resistant cell lines.

Abstract

A cDNA for threonyl-tRNA synthetase was isolated from a human placental cDNA lambda gt11 expression library by immunological screening, and its identity was confirmed by hybrid-selected mRNA translation. With this cDNA used as a hybridization probe, borrelidin-resistant Chinese hamster ovary cells that overproduced threonyl-tRNA synthetase were shown to have increased levels of threonyl-tRNA synthetase mRNA and gene sequences. Amplification of the gene did not appear to have been accompanied by any major structural reorganizations.

Entities: Chemical Gene Species

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Year: 1989 PMID： 2747635 PMCID： PMC362973 DOI： 10.1128/mcb.9.5.1832-1838.1989

Source DB: PubMed Journal: Mol Cell Biol ISSN： 0270-7306 Impact factor: 4.272

31 in total

1. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.

Authors: P Chomczynski; N Sacchi
Journal: Anal Biochem Date: 1987-04 Impact factor: 3.365

2. Structure of the yeast valyl-tRNA synthetase gene (VASI) and the homology of its translated amino acid sequence with Escherichia coli isoleucyl-tRNA synthetase.

Authors: X Jordana; B Chatton; M Paz-Weisshaar; J M Buhler; F Cramer; J P Ebel; F Fasiolo
Journal: J Biol Chem Date: 1987-05-25 Impact factor: 5.157

3. Overexpression of mammalian phenylalanyl-tRNA synthetase upon phenylalanine restriction.

Authors: M Lazard; M Mirande; J P Waller
Journal: FEBS Lett Date: 1987-05-25 Impact factor: 4.124

4. Expression of the aminoacyl-tRNA synthetase complex in cultured Chinese hamster ovary cells. Specific depression of the methionyl-tRNA synthetase component upon methionine restriction.

Authors: M Lazard; M Mirande; J P Waller
Journal: J Biol Chem Date: 1987-03-25 Impact factor: 5.157

5. Identification of 28 DNA fragments that detect RFLPs in 13 distinct physical regions of the short arm of chromosome 5.

Authors: J Overhauser; J McMahan; J J Wasmuth
Journal: Nucleic Acids Res Date: 1987-06-11 Impact factor: 16.971

Isolation of a cDNA clone for human threonyl-tRNA synthetase: amplification of the structural gene in borrelidin-resistant cell lines.

1. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.

2. Structure of the yeast valyl-tRNA synthetase gene (VASI) and the homology of its translated amino acid sequence with Escherichia coli isoleucyl-tRNA synthetase.

3. Overexpression of mammalian phenylalanyl-tRNA synthetase upon phenylalanine restriction.

4. Expression of the aminoacyl-tRNA synthetase complex in cultured Chinese hamster ovary cells. Specific depression of the methionyl-tRNA synthetase component upon methionine restriction.

5. Identification of 28 DNA fragments that detect RFLPs in 13 distinct physical regions of the short arm of chromosome 5.

6. A fine structure physical map of the short arm of chromosome 5.

7. Isolation, structure and expression of mammalian genes for histidyl-tRNA synthetase.

8. Threonyl-tRNA synthetase gene maps close to leucyl-tRNA synthetase gene on human chromosome 5.

9. Specific sequence homology and three-dimensional structure of an aminoacyl transfer RNA synthetase.

10. A general method for preparing chromatin containing intact DNA.

1. The human QARS locus: assignment of the human gene for glutaminyl-tRNA synthetase to chromosome 1q32-42.

2. Evidence that gene G7a in the human major histocompatibility complex encodes valyl-tRNA synthetase.