Literature DB >> 27470136

3D structure determination of a protein in living cells using paramagnetic NMR spectroscopy.

Bin-Bin Pan1, Feng Yang1, Yansheng Ye2, Qiong Wu2, Conggang Li2, Thomas Huber3, Xun-Cheng Su1.   

Abstract

Determining the three-dimensional structure of a protein in living cells remains particularly challenging. We demonstrated that the integration of site-specific tagging proteins and GPS-Rosetta calculations provides a fast and effective way of determining the structures of proteins in living cells, and in principle the interactions and dynamics of protein-ligand complexes.

Entities:  

Year:  2016        PMID： 27470136     DOI： 10.1039/c6cc05490k

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  20 in total

1.  A new carbamidemethyl-linked lanthanoid chelating tag for PCS NMR spectroscopy of proteins in living HeLa cells.

Authors:  Yuya Hikone; Go Hirai; Masaki Mishima; Kohsuke Inomata; Teppei Ikeya; Souichiro Arai; Masahiro Shirakawa; Mikiko Sodeoka; Yutaka Ito
Journal:  J Biomol NMR       Date:  2016-09-08       Impact factor: 2.835

2.  A protocol for the refinement of NMR structures using simultaneously pseudocontact shift restraints from multiple lanthanide ions.

Authors:  Davide Sala; Andrea Giachetti; Claudio Luchinat; Antonio Rosato
Journal:  J Biomol NMR       Date:  2016-10-22       Impact factor: 2.835

3.  Conformationally locked lanthanide chelating tags for convenient pseudocontact shift protein nuclear magnetic resonance spectroscopy.

Authors:  Daniel Joss; Roché M Walliser; Kaspar Zimmermann; Daniel Häussinger
Journal:  J Biomol NMR       Date:  2018-08-16       Impact factor: 2.835

4.  Preferential Regulation of Transient Protein-Protein Interaction by the Macromolecular Crowders.

Authors:  Zhou Gong; Ju Yang; Ling-Yun Qin; Chun Tang; Hanqiu Jiang; Yubin Ke; Xu Dong
Journal:  J Phys Chem B       Date:  2022-06-22       Impact factor: 3.466

Review 5.  In-Cell NMR Spectroscopy of Intrinsically Disordered Proteins.

Authors:  Nicholas Sciolino; David S Burz; Alexander Shekhtman
Journal:  Proteomics       Date:  2019-01-15       Impact factor: 3.984

Review 6.  NMR of Macromolecular Assemblies and Machines at 1 GHz and Beyond: New Transformative Opportunities for Molecular Structural Biology.

Authors:  Caitlin M Quinn; Mingzhang Wang; Tatyana Polenova
Journal:  Methods Mol Biol       Date:  2018

7.  In-cell destabilization of a homodimeric protein complex detected by DEER spectroscopy.

Authors:  Yin Yang; Shen-Na Chen; Feng Yang; Xia-Yan Li; Akiva Feintuch; Xun-Cheng Su; Daniella Goldfarb
Journal:  Proc Natl Acad Sci U S A       Date:  2020-08-11       Impact factor: 11.205

8.  Visualizing Proteins in Mammalian Cells by 19 F NMR Spectroscopy.

Authors:  Wenkai Zhu; Alex J Guseman; Fatema Bhinderwala; Manman Lu; Xun-Cheng Su; Angela M Gronenborn
Journal:  Angew Chem Int Ed Engl       Date:  2022-03-30       Impact factor: 16.823

Review 9.  Radio Signals from Live Cells: The Coming of Age of In-Cell Solution NMR.

Authors:  Enrico Luchinat; Matteo Cremonini; Lucia Banci
Journal:  Chem Rev       Date:  2022-01-21       Impact factor: 72.087

10.  Improved in-cell structure determination of proteins at near-physiological concentration.

Authors:  Teppei Ikeya; Tomomi Hanashima; Saori Hosoya; Manato Shimazaki; Shiro Ikeda; Masaki Mishima; Peter Güntert; Yutaka Ito
Journal:  Sci Rep       Date:  2016-12-02       Impact factor: 4.379
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